Seasonal changes in the molecular species and nuclear binding of the chick oviduct progesterone receptor.
Seasonal changes in the molecular species and nuclear binding of the chick oviduct progesterone receptor.
复制标题
鸡输卵管孕酮受体的分子种类和核结合的季节性变化。
DOI：
10.1021/bi00584a008
复制
发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
T. Spelsberg
中科院分区：
文献类型：
--
作者：
Patricia A. Boyd;T. Spelsberg
Patricia A. Boyd* 和托马斯C. Spelsberg* 摘要：已描述了雌激素处理的未成熟小鸡的发育输卵管中的孕酮受体复合物与分离的染色质和部分纯化的与脱氧核糖核酸（DNA）结合的受体蛋白结合的能力的季节性变化。在冬末分离的受体未能结合到这些网站，而在一年中的其余时间分离的受体显示出显着的结合。与DNA结合的水平全年保持不变。一个类似的季节性变化的核转位在体内的[3H]孕酮证明。此外，孕酮对体内内源性核糖核酸聚合酶II（B）活性的影响也显示出季节性变化。体内结合和转录效应的变化与体外结合具有相似的周期性和时序性。两种分子种类之一的水平
Patricia A. Boyd* and Thomas C. Spelsberg* abstract: A seasonal variation in the capacity of the progesterone-receptor complex to bind to isolated chromatin and partially purified acceptor protein bound to deoxyribonucleic acid (DNA) from the developed oviducts of estrogen-treated immature chicks has been described. Receptors isolated during the late winter fail to bind to these sites, whereas receptors isolated during the rest of the year show a marked binding. The level of binding to DNA remains unchanged throughout the year. A similar seasonal variation in the nuclear trans-location in vivo of [3H] progesterone is demonstrated. Further, the effect of progesterone on the endogenous ribonucleic acid polymerase II (B) activity in vivo also displays a seasonal variation. The variations in the in vivo binding and tran-scriptional effectsshow a similar periodicity and timing as the in vitro binding. Thelevel of one of the two molecular species