Infectivity of Chlamydia trachomatisSerovar LGV but Not E Is Dependent on Host Cell Heparan Sulfate
Infectivity of Chlamydia trachomatisSerovar LGV but Not E Is Dependent on Host Cell Heparan Sulfate
复制标题
沙眼衣原体血清型LGV而非E的感染性依赖于宿主细胞硫酸乙酰肝素
DOI：
10.1128/iai.69.2.968-976.2001
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发表时间：
2001
影响因子：
3.1
通讯作者：
A. Eley
中科院分区：
文献类型：
--
作者：
M. Taraktchoglou;A. Pacey;J. Turnbull;A. Eley
摘要：本文研究了硫酸肝素、肝素和其他糖胺聚糖对沙眼衣原体血清型E和LGV感染的抑制作用，并对来自人类女性生殖道的三种细胞类型（包括原代人输卵管内细胞）进行了简单的竞争性抑制实验。我们比较了野生型中国仓鼠卵巢细胞系和两种糖胺聚糖缺乏细胞系的衣原体感染能力。研究表明，LGV不能感染硫酸肝素缺乏和gag缺乏的中国仓鼠卵巢细胞系，而E血清型感染这些细胞的效率与对照（非缺陷）细胞一样高。这两组实验证实，血清型LGV比血清型e更依赖于与硫酸肝素相关的感染机制。这一事实进一步得到了支持，即试图从糖胺聚糖缺乏细胞系中培养的两种血清型中纯化硫酸肝素样分子均无效。以前的报道表明，衣原体能够产生一种类似硫酸肝素的分子，这种分子对附着和传染性很重要。我们已经尝试用流式细胞术检测一种特异性硫酸肝素抗体与沙眼衣原体的可能结合。结果显示，硫酸肝素抗体不与血清型沙眼衣原体LGV或e结合。我们的结果强烈表明，衣原体不产生硫酸肝素样分子，而是利用宿主细胞中的硫酸肝素来感染细胞。
ABSTRACT The ability of heparan sulfate, heparin, and other glycosaminoglycans to inhibit the infectivity of Chlamydia trachomatis serovars E and LGV was examined using a simple competitive inhibition assay with three cell types from the human female reproductive tract, including primary human endosalpingeal cells. With the majority of the glycosaminoglycans tested, LGV was more significantly inhibited than serovar E. We have compared chlamydial infectivity between a wild-type Chinese hamster ovary cell line and two glycosaminoglycan-deficient cell lines. LGV was shown to be unable to infect heparan sulfate-deficient and GAG-deficient Chinese hamster ovary cell lines, whereas the E serovar infected these cells as efficiently as the control (nondeficient) cells. These two sets of experiments confirmed that serovar LGV is more dependent on a heparan sulfate-related mechanism of infectivity than is serovar E. This is further supported by the fact that attempts to purify a heparan sulfate-like molecule from either serovar cultured in glycosaminoglycan-deficient cell lines were nonproductive. Previous reports have suggested that chlamydia are able to produce a heparan sulfate-like molecule that is important for attachment and infectivity. We have attempted to detect possible binding of a specific heparan sulfate antibody to C. trachomatis by flow cytometry. Results showed no binding of the heparan sulfate antibody to C. trachomatis serovar LGV or E. Our results strongly indicate that chlamydiae do not produce a heparan sulfate-like molecule but rather use host cell heparan sulfate in order to infect cells.
DOI：
10.1073/pnas.82.10.3197
发表时间：
1985-01-01
影响因子：
11.1
作者：
ESKO, JD;STEWART, TE;TAYLOR, WH
通讯作者：
TAYLOR, WH
小鼠阴道上皮中上皮分化和细胞表面硫酸乙酰肝素蛋白多糖表达的激素修饰。
DOI：
--
发表时间：
1988
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Hayashi,K;Hayashi,M;Boutin,E;Cunha,GR;Bernfield,M;Trelstad,RL
通讯作者：
Trelstad,RL
DOI：
10.1073/pnas.89.6.2267
发表时间：
1992-03-15
影响因子：
11.1
作者：
LIDHOLT, K;WEINKE, JL;ESKO, JD
通讯作者：
ESKO, JD