An ENA ATPase, MaENA1, of Metarhizium acridum influences the Na+-, thermo- and UV-tolerances of conidia and is involved in multiple mechanisms of stress tolerance
An ENA ATPase, MaENA1, of Metarhizium acridum influences the Na+-, thermo- and UV-tolerances of conidia and is involved in multiple mechanisms of stress tolerance
复制标题
Metarhizium acridum 的 ENA ATP 酶 MaENA1 影响分生孢子的 Na 耐受性、热耐受性和 UV 耐受性，并参与多种胁迫耐受机制
DOI：
10.1016/j.fgb.2015.08.011
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发表时间：
2015-10-01
影响因子：
3
通讯作者：
Xia, Yuxian
中科院分区：
文献类型：
--
作者：
Ma, Qinsi;Jin, Kai;Xia, Yuxian
在真菌中，ENA ATP酶在渗透和碱性pH耐受性中发挥关键作用，尽管它们在耐热和紫外耐受性中的功能尚未被探索。昆虫病原真菌在自然界广泛分布，在害虫防治中具有相当大的潜力。对昆虫病原真菌绿僵菌（Metarhiziumacridum）的ENA-ATPase基因MaENA 1进行了缺失分析。MaENA 1破坏菌株（Δ MaENA 1）比野生型菌株（WT）对NaCl、热和UV辐射的耐受性更低。分生孢子RNA的数字基因表达谱分析在WT和Delta MaENA 1菌株之间产生281个差异表达基因（DEG）。85个DEG，其中56个在Delta MaENA 1菌株中下调，被证明与热/紫外耐受性相关，包括6个细胞色素P450超家族基因，35个氧化还原酶基因，24个离子结合基因，7个DNA修复基因和5个其他基因。另外，Ras-cAMP PKA途径、RIM 101途径、Ca 2 +/钙调素途径、TOR途径和HOG/Spcl/Styl/JNK途径等8个基因参与了胁迫应答途径。这些结果表明MaENA 1影响了M. acridum，并参与多种胁迫耐受机制。因此，MaENA 1是昆虫病原真菌在环境和宿主的应激条件下适应和生存所必需的。(C)2015 Elsevier Inc. All rights reserved.
In fungi, ENA ATPases play key roles in osmotic and alkaline pH tolerance, although their functions in thermo- and UV-tolerances have not been explored. Entomopathogenic fungi are naturally widespread and have considerable potential in pest control. An ENA ATPase gene, MaENA1, from the entomopathogenic fungus Metarhizium acridum was functionally analyzed by deletion. MaENA1-disruption strain (Delta MaENA1) was less tolerant to NaCl, heat, and UV radiation than a wild-type strain (WT). Digital Gene Expression profiling of conidial RNAs resulted in 281 differentially expressed genes (DEGs) between the WT and Delta MaENA1 strains. Eighty-five DEGs, 56 of which were down-regulated in the Delta MaENA1 strain, were shown to be associated with heat/UV tolerance, including six cytochrome P450 superfamily genes, 35 oxidoreductase genes, 24 ion-binding genes, seven DNA repair genes, and five other genes. In addition, eight genes were components of stress responsive pathways, including the Ras-cAMP PKA pathway, the RIM101 pathway, the Ca2+/calmodulin pathway, the TOR pathway, and the HOG/Spcl/Styl/JNK pathway. These results demonstrated that MaENA1 influences fungal tolerances to Na+, heat, and UV radiation in M. acridum, and is involved in multiple mechanisms of stress tolerance. Therefore, MaENA1 is required for the adaptation and survival of entomopathogenic fungi in stressful conditions in the environment and in their hosts. (C) 2015 Elsevier Inc. All rights reserved.