ABUNDANCE OF VIRUSES IN MARINE WATERS - ASSESSMENT BY EPIFLUORESCENCE AND TRANSMISSION ELECTRON-MICROSCOPY
ABUNDANCE OF VIRUSES IN MARINE WATERS - ASSESSMENT BY EPIFLUORESCENCE AND TRANSMISSION ELECTRON-MICROSCOPY
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DOI：
10.1128/aem.57.9.2731-2734.1991
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发表时间：
1991-09-01
影响因子：
4.4
通讯作者：
KOIKE, I
中科院分区：
文献类型：
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作者：
HARA, S;TERAUCHI, K;KOIKE, I
使用孔径为 0.015 微米的 Nuclepore 过滤器的落射荧光显微镜评估了日本沿海和近海水域上层细菌和微小 DNA 相关颗粒的丰度。 将微小 DNA 相关颗粒的数量与透射电子显微镜估计的病毒颗粒的丰度进行比较。 虽然通过透射电子显微镜方法获得的病毒丰度差异较大（1.2 x 10(6)至35 x 10(6) ml-1），但4',6-二脒基-2-苯基吲哚反应性微小颗粒与病毒的比例在相当窄的范围内（1.0至1.6），表明落射荧光显微镜鉴定的大多数与DNA相关的微小颗粒实际上是病毒颗粒。 这一结果意味着使用落射荧光显微镜来评估海洋环境中病毒丰度的可能性。
Abundance of bacteria and tiny DNA-associated particles in the upper layer of Japanese coastal and offshore waters was evaluated by epifluorescence microscopy with 0.015-mu-m-pore-size Nuclepore filters. The number of tiny DNA-associated particles was compared with the abundance of virus particles estimated by transmission electron microscopy. Although a large variation in virus abundance (1.2 x 10(6) to 35 x 10(6) ml-1) was obtained with the transmission electron microscopy method, the ratio of 4',6-diamidino-2-phenylindole-reactive tiny particles to viruses was in a rather narrow range (1.0 to 1.6), indicating that the majority of the tiny DNA-associated particles identified by epifluorescence microscopy were actually virus particles. This result implies the possibility of using epifluorescence microscopy for the evaluation of virus abundance in marine environments.