Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor.
Monitoring of transplanted liver health by quantification of organ-specific genomic marker in circulating DNA from receptor.
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DOI：
10.1371/journal.pone.0113987
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Rubio A
中科院分区：
文献类型：
--
作者：
Macher HC;Suárez-Artacho G;Guerrero JM;Gómez-Bravo MA;Álvarez-Gómez S;Bernal-Bellido C;Dominguez-Pascual I;Rubio A
由于关系到器官移植受者的长期生存和器官健康维护，移植器官的健康评估非常重要。如今，测量移植受者循环中移植物的游离 DNA 已被认为是器官排斥或移植相关并发症的潜在生物标志物，试图取代或减少肝活检。然而，迄今为止开发的方法昂贵且极其耗时。我们的方法是在性别不匹配的男性捐赠器官女性接受者中测量 SRY 基因作为男性器官生物标志物。在再灌注期间（第 0 天）移植时以及在重症监护病房期间，预先通过实时定量 PCR 进行 SRY 基因的无细胞 DNA 定量。 β-珠蛋白无细胞 DNA 水平（一种通用的细胞损伤标志物）也进行了定量。在术后第一周内接受移植且没有任何并发症的患者的 β 珠蛋白平均值从第 0 天开始下降，直至患者稳定。在患有某种移植后并发症的患者中，这种下降并不那么明显。所有患者的 SRY 水平在第 0 天均有所增加，但在住院期间有所下降。不损害捐献器官的不同并发症显示β-珠蛋白水平升高，但没有SRY基因水平。然而，当捐赠的器官受损时，患者会表现出高水平的这两种基因。测定女性受者血清中的 SRY 基因是捐赠器官的明确且特异的生物标志物，可以通过非昂贵的技术在短时间内为我们提供有关移植物健康状况的重要信息。这种方法可以让临床医生对移植患者进行密切随访。
Health assessment of the transplanted organ is very important due to the relationship of long-term survival of organ transplant recipients and health organ maintenance. Nowadays, the measurement of cell-free DNA from grafts in the circulation of transplant recipients has been considered a potential biomarker of organ rejection or transplant associated complications in an attempt to replace or reduce liver biopsy. However, methods developed to date are expensive and extremely time-consuming. Our approach was to measure the SRY gene, as a male organ biomarker, in a setting of sex-mismatched female recipients of male donor organs. Cell-free DNA quantization of the SRY gene was performed by real-time quantitative PCR beforehand, at the moment of transplantation during reperfusion (day 0) and during the stay at the intensive care unit. Beta-globin cell-free DNA levels, a general cellular damage marker, were also quantified. Beta-globin mean values of patients, who accepted the graft without any complications during the first week after surgery, diminished from day 0 until patient stabilization. This decrease was not so evident in patients who suffered some kind of post-transplantation complications. All patients showed an increase in SRY levels at day 0, which decreased during hospitalization. Different complications that did not compromise donated organs showed increased beta-globin levels but no SRY gene levels. However, when a donated organ was damaged the patients exhibited high levels of both genes. Determination of a SRY gene in a female recipient's serum is a clear and specific biomarker of donated organs and may give us important information about graft health in a short period of time by a non-expensive technique. This approach may permit clinicians to maintain a close follow up of the transplanted patient.
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影响因子：
9.3
作者：
Beck, Julia;Bierau, Sarah;Schuetz, Ekkehard
通讯作者：
Schuetz, Ekkehard
影响因子：
5
作者：
Macher, Hada;Egea-Guerrero, Juan J.;Rubio, Amalia
通讯作者：
Rubio, Amalia
影响因子：
3.9
作者：
Mortarino, M.;Garagiola, I.;Peyvandi, F.
通讯作者：
Peyvandi, F.
影响因子：
5
作者：
Macher, Hada C.;Noguerol, Pilar;Guerrero, Juan M.
通讯作者：
Guerrero, Juan M.
影响因子：
9.3
作者：
Rainer, TH;Wong, LKS;Lo, YMD
通讯作者：
Lo, YMD