Improving the acetylcholinesterase inhibitory effect of &ITIlligera henryi&IT by solid-state fermentation with &ITClonostachys rogersoniana&IT
Improving the acetylcholinesterase inhibitory effect of &ITIlligera henryi&IT by solid-state fermentation with &ITClonostachys rogersoniana&IT
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提高乙酰胆碱酯酶抑制作用
DOI：
10.1016/j.jbiosc.2017.05.012
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发表时间：
2017-11-01
影响因子：
2.8
通讯作者：
Ding, Zhong-Tao
中科院分区：
文献类型：
--
作者：
Li, Xue-Jiao;Dong, Jian-Wei;Ding, Zhong-Tao
百合是一种地方性中药，含有丰富的阿啡类生物碱，具有多种生物活性。本研究用几种真菌对亨利块茎进行发酵，测定了未发酵和发酵的亨利块茎乙酰胆碱酯酶（AChE）抑制活性。结果表明，用rogersoniana Clonostachys 828H2发酵乳酸菌能有效提高乳酸菌乙酰胆碱酯酶（AChE）抑制活性。在C. rogersoniana发酵过程中发现了一个关键的生物转化，以阐明对亨利氏酵母乙酰胆碱酯酶抑制活性的提高：(S)-actinodaphnine(1)转化为新的4-hydroxyaporphine生物碱(4R,6aS)-4-hydroxyactinodaphnine(2)，具有较强的AChE抑制活性，IC50值为17.66 +/- 0.06 μ M.本文首次报道了对麻霉进行纯菌种发酵处理，提示C. rogersoniana发酵可能是一种潜在的麻霉加工方法。(C) 2017，生物技术学会，日本。版权所有。
llligera henryi, an endemic traditional Chinese medicine, contains abundant aporphine alkaloids that possess various bioactivities. In the present study, tubers of I. henryi were fermented by several fungi, and the acetylcholinesterase (AChE) inhibitory activities of non-fermented and fermented I. henryi were measured. The results showed that the fermentation of l. henryi with Clonostachys rogersoniana 828H2 is effective for improving the AChE inhibitory activity. A key biotransformation was found during the C. rogersoniana fermentation for clarifying the improvement of the AChE inhibitory activity of I. henryi: (S)-actinodaphnine (1) was converted to a new 4-hydroxyaporphine alkaloid (4R,6aS)-4-hydroxyactinodaphnine (2) that possessed a stronger AChE inhibitory activity, with an IC50 value of 17.66 +/- 0.06 mu M. This paper is the first to report that the pure strain fermentation processing of f. henryi and indicated C. rogersoniana fermentation might be a potential processing method for J. henryi. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.