Motility efficiency and spatiotemporal synchronization in non-metastatic vs. metastatic breast cancer cells
Motility efficiency and spatiotemporal synchronization in non-metastatic vs. metastatic breast cancer cells
复制标题
DOI：
10.1039/c3ib40144h
复制
发表时间：
2013-01-01
影响因子：
2.5
通讯作者：
Grzybowski, Bartosz A.
中科院分区：
文献类型：
--
作者：
Hermans, Thomas M.;Pilans, Didzis;Grzybowski, Bartosz A.
转移性乳腺癌细胞不仅比其非转移性变体移动得更快和更持久，而且在这样做时更有效地利用了细胞骨架的机械功。细胞运动的效率是针对整个细胞（而不是细胞膜的部分）定义的，并且与形成膜突起和缩回所花费的功有关。反过来，这项工作是通过整合沿着整个细胞周边的突出和收缩区域来估计的，并相对于细胞的净易位进行标准化。然后使用互相关、格兰杰因果关系和形态动力学分析的组合将效率与细胞膜动力学相关联。在转移性细胞中，突起和缩回在空间和时间上都是高度“同步”的，并且这些细胞有效地移动。相比之下，由非转移性细胞形成的突起和缩回不是“同步的”，对应于低运动效率。我们的工作提供了细胞运动的运动学和它们的能量学之间的联系。这也表明时空同步可能是癌细胞侵袭性的标志之一。
Metastatic breast cancer cells move not only more rapidly and persistently than their non-metastatic variants but in doing so use the mechanical work of the cytoskeleton more efficiently. The efficiency of the cell motions is defined for entire cells (rather than parts of the cell membrane) and is related to the work expended in forming membrane protrusions and retractions. This work, in turn, is estimated by integrating the protruded and retracted areas along the entire cell perimeter and is standardized with respect to the net translocation of the cell. A combination of cross-correlation, Granger causality, and morphodynamic profiling analyses is then used to relate the efficiency to the cell membrane dynamics. In metastatic cells, the protrusions and retractions are highly "synchronized'' both in space and in time and these cells move efficiently. In contrast, protrusions and retractions formed by non-metastatic cells are not "synchronized'' corresponding to low motility efficiencies. Our work provides a link between the kinematics of cell motions and their energetics. It also suggests that spatiotemporal synchronization might be one of the hallmarks of invasiveness of cancerous cells.