T-cell proliferative response to hapten-modified self-immunoglobulins: recognition of conjugate-specific determinants.
T-cell proliferative response to hapten-modified self-immunoglobulins: recognition of conjugate-specific determinants.
复制标题
T 细胞对半抗原修饰的自身免疫球蛋白的增殖反应：缀合物特异性决定簇的识别。
DOI：
10.1073/pnas.79.13.4156
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发表时间：
1982
影响因子：
11.1
通讯作者：
Bikoff,E
中科院分区：
文献类型：
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作者：
Bikoff,E
（4-羟基-3-硝基苯基）乙酰基（NP）修饰的BALB/c免疫球蛋白被用作诱导BALB/c小鼠增殖反应的免疫原。与对其他可溶性抗原的反应一样，增殖依赖于Lyt-1细胞和组织相容性抗辐射辅助细胞。针对NP修饰的自身免疫球蛋白的Lyt-1细胞对免疫半抗原NP具有特异性。然而，它们不识别半抗原本身。相反，他们看到了由半抗原NP和免疫球蛋白自身载体组成的复杂决定簇。通过将相同的半抗原偶联至不同的单克隆BALB/c抗体分子或通过经由间隔分子将半抗原附接至免疫球蛋白自身载体来产生不同的特异性。有人提出，通过将半抗原附着到自身免疫球蛋白分子上产生的决定簇与抗独特型T细胞识别的决定簇相似。
(4-Hydroxy-3-nitrophenyl)acetyl (NP)-modified BALB/c immunoglobulins were used as the immunogen for induction of a proliferative response in BALB/c mice. As is true for responses to other soluble antigens, proliferation was dependent on Lyt-1 cells and histocompatible radioresistant accessory cells. Lyt-1 cells directed against NP-modified self immunoglobulin are specific for the immunizing hapten NP. However, they do not recognize hapten per se. Rather, they see complex determinants comprised of both the hapten NP and the immunoglobulin self-carrier. Distinct specificities were created by coupling the same hapten to different monoclonal BALB/c antibody molecules or by attaching the hapten to the immunoglobulin self-carrier via a spacer molecule. It is proposed that determinants created by attaching haptens to self-immunoglobulin molecules are similar to those recognized by anti-idiotypic T cells.