Quantitative FDG-uptake by positron emission tomography in progressive hypertrophy of rat hearts in vivo
Quantitative FDG-uptake by positron emission tomography in progressive hypertrophy of rat hearts in vivo
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DOI：
10.1007/s12149-007-0067-2
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发表时间：
2007-12-01
影响因子：
2.6
通讯作者：
Komeda, Masashi
中科院分区：
文献类型：
--
作者：
Handa, Nobuhiro;Magata, Yasuhiro;Komeda, Masashi
目的探讨心肌FDG-PET在大鼠进行性心肌肥厚过程中的应用价值。方法采用盐敏感性Dahl大鼠（Dahl-S）6只，以盐敏感性Dahl-S为对照组，以盐敏感性Dahl-S为对照组，以盐敏感性Dahl-S为对照组，以盐敏感性Dahl-S为对照组。抗性Dahl大鼠（对照）。分别在心肌肥厚早期（ES：14-18周）和晚期（LS：22-26周）应用FDG-PET两次。计算标准摄取值（SUV），比较不同动物体重和不同FDG注射剂量之间的差异。为了验证定量研究，共36个组织样本的放射性与相应的PET values.Results左心室质量Dahl-S增加了17%，在ES和LS的25%。Dahl-S的SUV在ES时为对照的95%，在LS时降低至62%（P = 0.023）。LS后心功能开始恶化。线性回归分析显示，组织样品放射性活度与PET值之间有良好的相关性（Y = 1.20X，P < 0.0001，R-2 = 0.979）。结论小动物体内多时段纵向PET研究是可行的，可用于葡萄糖摄取的重复性评价。随着时间的推移，进行性心脏肥大中葡萄糖摄取的减少可能先于其进展为心力衰竭。
Background Quantitative myocardial fluorodeoxyglucose positron emission tomography (FDG-PET) for assessing glucose uptake in vivo is reliable in normal rat heart.Objective To assess the applicability of myocardial FDG-PET on multiple occasions in the longitudinal disease process of progressive hypertrophy of rat heart.Methods Six salt-sensitive Dahl rats (Dahl-S) developing progressive hypertrophy with subsequent dilated cardiomyopathy were compared with salt-resistant Dahl rats (controls). FDG-PET was applied twice at early stage (ES: 14-18 weeks) and at late stage (LS: 22-26 weeks) of hypertrophy. Standardized uptake value (SUV) was calculated for comparing between different animal weights and different injection dosages of FDG. For validating the quantitative study, radioactivity of a total of 36 tissue samples was compared with the corresponding PET values.Results The left ventricular mass in Dahl-S increased by 17% at ES and by 25% at LS. The SUV in Dahl-S was 95% of controls at ES and reduced to 62% at LS (P = 0.023). The heart function started to deteriorate after LS. Linear regression analysis showed a good correlation between the radioactivity of tissue samples and PET values (Y = 1.20X, P < 0.0001, R-2 = 0.979).Conclusions Small animal PET studies on longitudinal multiple occasions in vivo were feasible and useful for the repeating assessment of glucose uptake. The reduction of glucose uptake in progressive hypertrophy of heart over time may precede its progression to heart failure.