Effect of temperature on host response to Batrachochytrium dendrobatidis infection in the mountain yellow-legged frog (Rana muscosa)
Effect of temperature on host response to Batrachochytrium dendrobatidis infection in the mountain yellow-legged frog (Rana muscosa)
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DOI：
10.7589/0090-3558-44.3.716
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发表时间：
2008-07-01
影响因子：
1.3
通讯作者：
Briggs, Cheryl J.
中科院分区：
文献类型：
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作者：
Andre, Sara E.;Parker, John;Briggs, Cheryl J.
引起壶菌病的致病性壶菌 Batrachochytrium dendrobatidis 与世界各地两栖动物种群数量的减少有关，其中包括加州内华达山脉当地黄腿蛙（Rana muscosa）种群的减少和灭绝。先前的研究表明，在17-25℃的温度范围内，树蛙芽孢杆菌在培养物中达到最大生长速度，并且暴露于非常高的温度可以清除青蛙的树突芽孢杆菌感染。在这里，我们展示了一项实验室实验的结果，其中受实验感染的 R. muscosa 蝌蚪在 17 和 22 C 的温度下经历变态。所有受感染的动物在相似的时间内都出现了临床疾病。然而，饲养在22℃的青蛙死亡率明显低于饲养在17℃的青蛙。在变态后35天内，饲养在22℃的青蛙有50%死亡，而饲养在17℃的青蛙则有95%死亡。尽管感染持续存在，但在 22°C 时，存活的青蛙的临床症状已消退。因为这两个温度都在树状芽孢杆菌生长的最佳温度范围内。我们认为结果的差异表明温度对宿主对壶菌病的抵抗力的影响，而不是单独对真菌的影响。
The pathogenic chytrid fungus Batrachochytrium dendrobatidis, which causes the disease chytridiomycosis, has been implicated in declines of amphibian populations throughout the world, including declins and extionctions of local populations of mountain yellow-legged frogs, Rana muscosa, in the California Sierra Nevada. Previous studies have shown B. dendrobatidis achieves its maximum grown rate in culture in the temperature range of 17-25 C, and exposure to very high temperatures can clear frogs of B. dendrobatitis infection. Here we present the results of a laboratory experiment in which experimentally infected R. muscosa tadpoles were followed through metamorphosis at temperatures of 17 and 22 C. All infected animals developed clinical disease within a simlar time frame. However, frogs housed at 22 C exhibited a significanly lower mortality than those housed at 17 C. Within 35 days after metamorphosis, 50% of the frogs housed at 22 C died, while 95% of the frogs housed at 17 C died. Clinical signs subsided in the sruviving frogs at 22 C, despite persistent infection. Because both temperatures are within the optimal thermal range for groth of B. dendrobatidis. We propose that the difference in outcome indicated the effect of temperature on the host's resistance to chytridiomycosis, rather than an effect on the fungus alone.