Effects of coenzyme Q(10) on LDL oxidation in vitro.
Effects of coenzyme Q(10) on LDL oxidation in vitro.
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发表时间：
2013
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通讯作者：
H. Ahmadvand;H. Mabuchi;A. Nohara;Junji Kobayahi;M. Kawashiri
中科院分区：
文献类型：
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作者：
H. Ahmadvand;H. Mabuchi;A. Nohara;Junji Kobayahi;M. Kawashiri
辅酶Q(10)是一种脂溶性苯醌，在心脏组织中具有良好的抗氧化性和膜稳定性。本研究定量研究了辅酶Q(10)对体外低密度脂蛋白氧化的影响。共轭双烯的形成、丙二醛(MDA)和电泳率分别作为低密度脂蛋白氧化的标志。辅酶Q(10)在体外可明显减少共轭双烯和丙二醛的生成。辅酶Q(10)也降低了氧化的人低密度脂蛋白的电泳率。结果表明，辅酶Q(10)在CuSO(4)介导的体外氧化低密度脂蛋白(P<；0.05)中显示出较强的抗氧化活性。辅酶Q(10)对低密度脂蛋白氧化的抑制作用在20~300 nM浓度范围内呈剂量依赖性。此外，还比较了辅酶Q(10)与维生素E和维生素C对低密度脂蛋白氧化的影响。这项研究表明，辅酶Q(10)在体外是一种有效的抗氧化剂，可以保护低密度脂蛋白免受氧化，可能是降低动脉粥样硬化、冠心病和其他自由基相关健康问题的良好替代方案。
Coenzyme Q(10), a lipid soluble benzoquinone, has excellent antioxidant and membrane stabilizing properties in the cardiac tissue. The present study quantitatively investigated the effects of coenzyme Q(10) on in vitro LDL oxidation. The formation of conjugated dienes, malondialdehyde (MDA) and electrophoretic mobility were monitored as markers of the oxidation of LDL, respectively. Coenzyme Q(10) showed a decrease the formation of conjugated dienes and MDA, respectively, against oxidation in vitro. Coenzyme Q(10) also reduced electrophoretic mobility of the oxidized human LDL. Thus, results showed that the coenzyme Q(10) exhibits strong antioxidant activity in CuSO(4)-mediated oxidation of LDL (P<0.05) in vitro. The inhibitory effects of the coenzyme Q(10) on LDL oxidation were dose-dependent at concentrations ranging from 20 nM to 300 nM. Moreover, the effects of coenzyme Q(10) on LDL oxidation were compared with vitamin E and vitamin C. This study showed that coenzyme Q(10) is a potent antioxidant to protect LDL against oxidation in vitro and may be a good alternative to reduce the risk of atherosclerosis and coronary heart disease and other free radical associated health problems.