A pragmatic guide to multiphoton microscope design.
A pragmatic guide to multiphoton microscope design.
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DOI：
10.1364/aop.7.000276
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发表时间：
2015-06-30
影响因子：
27.1
通讯作者：
Squier J
中科院分区：
文献类型：
--
作者：
Young MD;Field JJ;Sheetz KE;Bartels RA;Squier J
多光子显微镜已经成为研究微观结构和功能的普遍工具，涉及广泛的学科。因此，本文的目的是为多光子显微镜的构建和性能评估提供一个全面的资源，这对于目前正在开发或希望开始开发这项强大技术的广泛科学领域来说是可以理解的。考虑到这一点，我们开发了一个指南，以帮助设计一个多光子显微镜。我们讨论了光源的选择、色散的光学管理、带有扫描光学的图像中继系统、物镜的选择、单元件光收集理论、光子计数检测、图像渲染，最后，一个示例显微镜的构建指南。
Multiphoton microscopy has emerged as a ubiquitous tool for studying microscopic structure and function across a broad range of disciplines. As such, the intent of this paper is to present a comprehensive resource for the construction and performance evaluation of a multiphoton microscope that will be understandable to the broad range of scientific fields that presently exploit, or wish to begin exploiting, this powerful technology. With this in mind, we have developed a guide to aid in the design of a multiphoton microscope. We discuss source selection, optical management of dispersion, image-relay systems with scan optics, objective-lens selection, single-element light-collection theory, photon-counting detection, image rendering, and finally, an illustrated guide for building an example microscope.