BACTERIOPHAGE PMB12 CONVERSION OF SPORULATION DEFECT IN RNA-POLYMERASE MUTANTS OF BACILLUS-SUBTILIS
BACTERIOPHAGE PMB12 CONVERSION OF SPORULATION DEFECT IN RNA-POLYMERASE MUTANTS OF BACILLUS-SUBTILIS
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DOI：
10.1128/jvi.24.1.194-200.1977
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发表时间：
1977-01-01
影响因子：
5.4
通讯作者：
LOVETT, PS
中科院分区：
文献类型：
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作者：
BRAMUCCI, MG;KEGGINS, KM;LOVETT, PS
根据PMB 12能提高B的产孢率的特性，从土壤中分离得到了一株假温性噬菌体PMB 12。subtilis 168. PMB 12随后显示出在2种遗传上不同的孢子形成突变体中转化孢子形成缺陷。一类包括那些也是孢子阴性的利福平抗性突变体（在rif基因座突变）。另一类包括携带孢子形成突变spoCM-1的菌株。spoCM-1突变通过PBS 1转导与cysA 14相关，但与rif基因座不同。其他几种孢子形成突变体未被PMB 12转化。PMB 12与噬菌体PBS 1相关。PBS 1不能转化上述孢子形成突变体。PBS 2是PBS 1的透明空斑衍生物，其复制对利福平不敏感，这显然是由于噬菌体诱导的对利福平不敏感的RNA聚合酶。PMB 12复制也对利福平不敏感。
The pseudotemperate phage PMB12 was isolated from soil on the basis of its ability to enhance the rate of sporulation of B. subtilis 168. PMB12 was subsequently shown to convert the sporulation defect in 2 genetically distinct classes of sporulation mutants. One class includes those rifampin-resistant mutants that are also spore-negative (mutated at the rif locus). The other class includes a strain carrying the sporulation mutation spoCM-1. The spoCM-1 mutation is linked to cysA14 by PBS1 transduction but is distinct from the rif locus. Several other sporulation mutants were not converted by PMB12. PMB12 is related to phage PBS1. PBS1 did not convert the above sporulation mutants. The replication of PBS2, a clear-plaquing derivative of PBS1, is rifampin insensitive, apparently due to a phage-induced rifampin-insensitive RNA polymerase. PMB12 replication is also rifampin insensitive.