Efficacy of Photodynamic Anti‐Microbial Chemotherapy for Acanthamoeba Keratitis In Vivo
Efficacy of Photodynamic Anti‐Microbial Chemotherapy for Acanthamoeba Keratitis In Vivo
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体内光动力抗微生物化疗治疗棘阿米巴角膜炎的疗效
DOI：
10.1002/lsm.23355
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发表时间：
2020
影响因子：
2.4
通讯作者：
Sakaguchi Takemasa
中科院分区：
文献类型：
--
作者：
Dwia Pertiwi Yunialthy;Chikama Taiichiro;Sueoka Kentaro;Ko Ji‐Ae;Kiuchi Yoshiaki;Onodera Makoto;Sakaguchi Takemasa
背景和目的阿米巴角膜炎是一种危及视力的感染性疾病，治疗困难，本研究的目的是评估TONS 504（阳离子二氢卟酚衍生物光敏剂）介导的体内光动力抗菌化学疗法（PACT）作为一种潜在的治疗阿米巴角膜炎的方法。研究设计/材料和方法通过用1 × 105/ml卡氏阿米巴孵育的软性接触镜诱导阿米巴角膜炎，将其放置在具有临时睑板缝合术的清创角膜上。将38只雄性日本白色家兔随机分为3组（正常眼、未处理组和处理组）。TONS 504以1 mg/ml滴眼液的形式给药，然后在感染性接触透镜暴露后7天角膜炎建立后进行发光二极管照射。给药后每3天在裂隙灯显微镜下评价所有动物，持续6天。根据荧光素染色检测的角膜上皮缺损、基质混浊水肿和角膜血管浸润确定临床评分。6天后，眼睛被摘除组织病理学analysis. ResultsThe治疗组感染的临床体征显着减少治疗后6天。组织学检查显示，58%的角膜基质纤维排列规则，有少量炎性细胞。然而，治疗组中42%的角膜显示出浸润的中性粒细胞和基质胶原纤维的不规则排列。ConclusionsOur TONS 504 ‐PACT在58%的兔模型中实现了角膜炎的完全恢复。需要进一步研究来确定TONS 504 ‐PACT治疗阿米巴角膜炎的最大有效性的条件。© 2020 Wiley Periodicals LLC
Background and ObjectivesAcanthamoebakeratitis is a sight‐threatening infectious disease that is difficult to treat. The aim of this study was to evaluate TONS504 (cationic chlorin derivative photosensitizer)‐mediated photodynamic antimicrobial chemotherapy (PACT)in vivoas a potential treatment forAcanthamoebakeratitis.Study Design/Materials and MethodsAcanthamoebakeratitis was induced by soft contact lenses incubated with 1 × 105/mlAcanthamoeba castellanii, which were placed over debrided corneas with temporary tarsorrhaphy. Thirty‐eight male Japanese white rabbits were randomly divided into three groups (normal eye, no treatment, and treatment groups). TONS504 was administered as eye drops at 1 mg/ml, followed by light‐emitting diode irradiation after the establishment of keratitis at 7 days after infectious contact lens exposure. All animals were evaluated under a slit‐lamp microscope every 3 days for 6 days after the treatment. Clinical scores based on corneal epithelial defects detected by fluorescein staining, stromal opacity edema, and vascular infiltration into the cornea were determined. After 6 days, the eyes were enucleated for histopathological analysis.ResultsClinical signs of infection in the treatment group were markedly reduced for up to 6 days after treatment. Histopathology showed a regular arrangement of stromal fibers and a small number of inflammatory cells in 58% of the corneas. However, 42% of corneas in the treatment group showed infiltrating neutrophils and irregular alignment of stromal collagen fibers.ConclusionsOur TONS504‐PACT achieved complete recovery from keratitis in 58% of the rabbit models. Further studies are required to determine the conditions for the maximal effectiveness of our TONS504‐PACT forAcanthamoebakeratitis. Lasers Surg. Med. © 2020 Wiley Periodicals LLC