Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells
Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells
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DOI：
10.1016/s0956-5663(02)00015-5
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发表时间：
2002-06-01
影响因子：
12.6
通讯作者：
Sigworth, FJ
中科院分区：
文献类型：
--
作者：
Klemic, KG;Klemic, JF;Sigworth, FJ
膜片钳方法在玻璃微电极和细胞膜之间建立高阻‘千兆’时，以非常高的分辨率测量膜电流(Pflugers Arch)。391(1981)85；神经元8(1992)605)。在这里，我们描述了硅橡胶，聚(二甲基硅氧烷)(PDMS)，首次用于膜片钳电极。PDMS是一种很有吸引力的膜片钳记录材料。它的介电损耗很低，并且可以微成型(Annu。马特牧师。SCI。28(1998)153)制成模仿玻璃微吸管尖端的形状。此外，PDMS的表面化学也可以改变以模拟玻璃的亲水性(J.Appl。波兰姆。SCI。14(1970)2499；阿明。马特牧师。SCI。28(1998)153)，从而允许对细胞膜进行高阻密封。我们提出了一种由PDMS隔板组成的平面电极几何结构，该隔板在电极和浴室之间密封了一个小孔。我们证明了平面PDMS贴片电极，在弹性表面氧化后，允许在非洲爪哇卵母细胞上进行膜片钳记录。我们的结果表明，利用PDMS电极的平面阵列进行高通量膜片钳记录是有潜力的。(C)2002 Elsevier Science B.V.保留所有权利。
The patch clamp method measures membrane currents at very high resolution when a high-resistance 'gigaseal' is established between the glass microelectrode and the cell membrane (Pflugers Arch. 391 (1981) 85; Neuron 8 (1992) 605). Here we describe the first use of the silicone elastomer, poly(dimethylsiloxane) (PDMS), for patch clamp electrodes. PDMS is an attractive material for patch clamp recordings. It has low dielectric loss and can be micromolded (Annu. Rev. Mat. Sci. 28 (1998) 153) into a shape that mimics the tip of the glass micropipette. Also, the surface chemistry of PDMS may be altered to mimic the hydrophilic nature of glass (J. Appl. Polym. Sci. 14 (1970) 2499; Amin. Rev. Mat. Sci. 28 (1998) 153), thereby allowing a high-resistance seal to a cell membrane. We present a planar electrode geometry consisting of a PDMS partition with a small aperture sealed between electrode and bath chambers. We demonstrate that a planar PDMS patch electrode, after oxidation of the elastomeric surface, permits patch clamp recording on Xenopus oocytes. Our results indicate the potential for high-throughput patch clamp recording with a planar array of PDMS electrodes. (C) 2002 Elsevier Science B.V. All rights reserved.