Monitoring the Cellular Binding Events with Quartz Crystal Microbalance (QCM) Biosensors.
Monitoring the Cellular Binding Events with Quartz Crystal Microbalance (QCM) Biosensors.
复制标题
使用石英晶体微天平 (QCM) 生物传感器监测细胞结合事件。
DOI：
10.1007/978-1-4939-6911-1_21
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Zeng,Xiangqun
中科院分区：
文献类型：
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作者：
Rehman,Abdul;Zeng,Xiangqun
石英晶体微天平（QCM）生物传感器已被证明是一种无创和无标签的细胞测量工具。然而，由细胞和相关液体环境组成的生物膜的复杂性阻碍了在增加的质量和频率输出信号的变化之间形成明确的关系。因此，界面设计（表面化学，相互作用机制和数据采集），数据解释和设备制造的协议都需要精细细化，以使这些生物传感器在现实生活中流行。特别是在从结合事件中得出正确推论的意义上，流体效应（主要以QCM的阻尼阻力形式可见）应该从结合测量中定量地排除。这样的策略甚至可以跟踪细胞相互作用，而细胞相互作用是许多生命生理功能的基础，可以内置到智能功能设备中，用于即时诊断。本章提供了有关这些策略的技术细节，重点介绍了使用QCM方法测量抗CD-20抗体（利妥昔单抗）与b -淋巴瘤细胞相互作用的实验细节和程序。除了详细描述具体的相互作用外，我们还提供了数据解释和设备开发的机制，这些机制具有潜在的应用于其他技术。
Quartz crystal microbalance (QCM) biosensors have been demonstrated as noninvasive and label-free tools for cell based measurements. However, the complexity of biofilms composed of cells with the associated liquid environments is preventive towards forming explicit relationship between the added mass and the change in the frequency output signal. Therefore, the protocols of interface design (surface chemistry, interaction mechanism, and data acquisition), data interpretation, and device fabrication, all need to be finely refined in order to make these biosensors prevail in real life. Especially in the sense of deriving correct inferences from binding events, the fluidic effects (mostly visible in the form of damping resistance of QCM) should be quantitatively excluded from binding measurements. Such strategies can then track even the cellular interactions which are the basis of many physiological functions of life and can be built into smart functional devices for point of care diagnostics. This chapter provides technical details regarding these strategies with a focus on experimental details and procedures of the measurements of anti CD-20 antibody (Rituximab) interactions with B-Lymphoma cancer cells using the QCM method. In addition to a detailed description of specific interactions, we provide mechanisms of data interpretation and device development having potential application to other techniques.