Molecular and Functional Characterization of Canac035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)
Molecular and Functional Characterization of Canac035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)
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Canac035（一种来自辣椒（Capsicum annuum L.）的 NAC 转录因子）的分子和功能表征
DOI：
10.3389/fpls.2020.00014
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Rugang Chen
中科院分区：
文献类型：
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作者：
Huafeng Zhang;Fang Ma;Xinke Wang;Suya Liu;Ul Haq Saeed;Xiaoming Hou;Yumeng Zhang;Dan Luo;Yuancheng Meng;Wei Zhang;Khan Abid;Rugang Chen
NAC（NAM，ATAF 1/2，CUC 2）蛋白是植物特异性转录因子，在植物应答非生物胁迫中起重要作用。然而，关于NAC在辣椒非生物胁迫耐受性中所起的功能作用的知识是有限的。在本研究中，我们从辣椒（Capsicum annuum L.）其中蛋白质定位于细胞核中并作为转录激活剂发挥作用。CaNAC 035的表达受低温和高温、渗透胁迫、盐、赤霉酸诱导。(GA)、甲基茉莉酸（MeJA）、水杨酸（SA）和脱落酸（阿坝）。为了了解CaNAC 035在非生物胁迫反应中的功能，我们利用病毒诱导的辣椒基因沉默来敲除CaNAC 035，并在拟南芥中过表达CaNAC 035。结果表明，CaNAC 035基因沉默的辣椒幼苗在低温、NaCl和甘露醇处理后，表现出比对照更严重的伤害。低温处理后，电解质渗漏率、丙二醛（MDA）、过氧化氢（H_2O_2）和超氧阴离子自由基（O_2）水平也相应增加。CaNAC 035基因沉默的拟南芥幼苗叶绿素含量较低，而CaNAC 035基因过表达的拟南芥幼苗在甘露醇和NaCl处理后的发芽率和鲜重均较高。我们还报道了18个可能与CaNAC 035相互作用的蛋白质，这些蛋白质可能参与胁迫反应、抗性和光合作用等过程。我们的研究结果表明，CaNAC 035是辣椒非生物胁迫耐受性的一个正调节因子，它通过多种信号途径起作用。
NAC (NAM, ATAF1/2, and CUC2) proteins are the plant-specific transcription factors (TFs).which are important in plant response to abiotic stresses. However, knowledge about the.functional role that NACs play in pepper abiotic stress tolerance is limited. In this study, we.isolated a NAC TF gene, CaNAC035, from pepper (Capsicum annuum L.), where the.protein is localized in the nucleus and functions as a transcriptional activator. CaNAC035.expression is induced by low and high temperatures, osmotic stress, salt, gibberellic acid.(GA), methyl-jasmonic acid (MeJA), salicylic acid (SA), and abscisic acid (ABA). To.understand the function of CaNAC035 in the abiotic stress responsep, we used virusinduced gene silencing in pepper to knockdown the CaNAC035 and overexpressed the.CaNAC035 in Arabidopsis. The results showed that pepper seedlings in which.CaNAC035 was silenced, showed more damage than the control pepper plants after.cold, NaCl, and mannitol treatments. Correspondingly increased electrolyte leakage, a.higher level of malondialdehyde (MDA), H2O2, and superoxide radicals were found after.cold treatments. CaNAC035-silenced seedlings exhibited lower chlorophyll content while.CaNAC035-overexpressed Arabidopsis plants had higher germination rate and fresh.weight after mannitol and NaCl treatments. We also reported 18 proteins that potentially.interact with CaNAC035 and may participate in processes such as the stress response,.resistance, and photosynthesis. Our results suggest that CaNAC035 is a positive.regulator of abiotic stress tolerance in pepper which acts through multiple.signaling pathways.