Synthetic'members of the folinic acid group.
Synthetic'members of the folinic acid group.
复制标题
亚叶酸族的合成成员。
DOI：
10.1021/ja01162a556
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发表时间：
1950
影响因子：
15
通讯作者：
L. Rogers
中科院分区：
文献类型：
--
作者：
W. Shive;T. Bardos;T. J. Bond;L. Rogers
Z.生理化学，214，281（1933），规定加热到50以加速二氧化碳的释放。Instituí für organische Chemie der Tbchnischbn Hochschulb达姆施塔特达姆施塔特，德国Clemens Schópf于1950年5月4日由尿素酶2接收，包含在一种比缩二脲更易溶于水的化合物中。用水萃取表明，这种物质在缩二脲中是以固溶体而不是单独的晶体存在。从甲醇经过繁琐的分步结晶后，反复得到熔点在110 ~ 115 ℃的材料，加入尿素后熔点有所降低。对典型制剂的分析得出N，44.6; C，21.2; H，6.2，但组成似乎因纯化方法而略有不同。它的分子量，由在水中的冰点降低法测定，范围从70到85。尿素酶的总水解产生33 - 41%的氮含量。在Nujol混悬液中获得的红外光谱与尿素的红外光谱无法区分。虽然上述数据中有一些与这一结论不一致，但对这些数据最有可能的解释是，分离出的物质不是纯化合物，而是尿素和具有类似元素组成的其他物质的松散化合物或固溶体。因此，迄今为止，我们的研究结果并不能证明除了尿素之外，还存在另一种脲酶底物。
Z. physiol. Chem·., 214, 281 (1933), subsequentheating to 50 has been prescribed to speed liberation of carbon dioxide. Instituí für organische Chemie der Tbchnischbn Hochschulb Darmstadt Darmstadt, Germany Clemens Schópf Received May 4, 1950 by urease2 and which is contained in a compound that is more soluble in water than is biuret. Ex-traction with water suggests that this material is present in biuret as a solid solution rather than as separate crystals. After tedious fractional crys-tallizations fromalcohol a material has been re-peatedly obtained with a rather sharp melting point at 110 to 115, which is somewhat lowered by additionsof urea. Analysis of a typical preparation gave N, 44.6; C, 21.2; H, 6.2, but the composition appearsto vary slightly with the method of purification. Its molecular weight, determined by thefreezing point lowering method in water, ranges from 70 to 85. Total hydrolysis by urease gives nitrogen content of 33 to 41%. Infrared spectrum, obtained in Nujol suspension, is indistinguishable from that of urea. Although some of the above data are inconsistent with this conclusion, the most probable interpretation of the data is that the isolated substance is not a pure chemical compound but is a loose compound or solid solution of urea and something else with a similar elementary composition. Therefore our results to date do not prove the existence of an-other substrate for urease besides urea.