Free radical scavengers, catalase and superoxide dismutase provide protection from oxalate-associated injury to LLC-PK1 and MDCK cells
Free radical scavengers, catalase and superoxide dismutase provide protection from oxalate-associated injury to LLC-PK1 and MDCK cells
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DOI：
10.1016/s0022-5347(05)67499-x
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发表时间：
2000-07-01
影响因子：
6.6
通讯作者：
Khan, SR
中科院分区：
文献类型：
--
作者：
Thamilselvan, S;Byer, KJ;Khan, SR
目的：目前的研究提供了证据表明，暴露于草酸盐和草酸钙晶体的肾上皮细胞诱导脂质过氧化和损伤细胞。由于氧化剂/抗氧化剂的平衡很可能发挥关键作用，我们确定了抗氧化剂清除剂的影响，对生产的自由基和损伤LLC-PK 1和MDCK细胞暴露于草酸盐（Ox）或Ox +草酸钙一水合物（COM）crystals.Materials和方法：LLC-PK 1和MDCK细胞生长在单层和暴露于1.0 mmol。Ox或1.0 mmol。牛+ 500微克/ ml. COM晶体120或240分钟。我们测定了乳酸脱氢酶（LDH）的释放作为细胞损伤的标志物和丙二醛（MDA）作为脂质过氧化的标志物。在存在或不存在400 U/ml的情况下测量超氧化物和羟基自由基。结果：OX处理后，LLC-PK 1细胞MDA含量和LDH释放量均显著增加，COM处理后，MDA含量和LDH释放量均显著增加。MDCK细胞对两种挑战的反应相似，但与LLC-PK 1细胞相比，其影响显著较小。这两种治疗方法都与这两种细胞类型的羟基和超氧自由基的产生显着增加。在这两种细胞系中，另外的过氧化氢酶或SOD显着降低MDA的增加和释放的LDH.Conclusions：本研究的结果表明，无论是Ox和COM晶体损伤肾上皮细胞和损伤与自由基的产生。近端小管起源的细胞比远端小管和集合管起源的细胞更敏感。自由基清除剂、过氧化氢酶和SOD提供显著保护。
Purpose: Current studies have provided evidence that exposure of renal epithelial cells to oxalate and calcium oxalate crystals induces lipid peroxidation and injures the cells. Since oxidant/antioxidant balance is likely to play a critical role, we determined the effect of antioxidant scavengers on production of free radicals and injury to LLC-PK1 and MDCK cells from exposure to oxalate (Ox) or Ox + calcium oxalate monohydrate (COM) crystals.Materials and Methods: LLC-PK1 and MDCK cells were grown in monolayers and exposed to 1.0 mmol. Ox or 1.0 mmol. Ox + 500 mu g./ml. COM crystals for 120 or 240 minutes. We measured the release of lactate dehydrogenase (LDH) as a marker for cell injury and malondialdehyde (MDA) as a marker of lipid peroxidation. Superoxide and hydroxyl radicals were measured in the presence or absence of 400 U/ml. catalase, or superoxide dismutase (SOD).Results: Exposure of LLC-PK1 cells to Ox resulted in a significant increase in MDA and release of LDH, which was further elevated when COM crystals were added. MDCK cells responded similarly to both challenges, but showed significantly less impact when compared with LLC-PK1 cells. Both treatments were associated with significant increase in the generation of hydroxyl and superoxide radicals by both cell types. In both cell lines, the addition of catalase or SOD significantly reduced the increase of MDA and release of LDH.Conclusions: Results of the present study indicate that both Ox and COM crystals are injurious to renal epithelial cells and the injury is associated with generation of free radicals. Cells of proximal tubular origin are more susceptible than those of distal tubules and collecting ducts. Free radical scavengers, catalase and SOD provide significant protection.