EFFECT OF INTRAVENTRICULAR-INJECTION OF AN ANTI-PURKINJE CELL ANTIBODY (ANTI-YO) IN A GUINEA-PIG MODEL
EFFECT OF INTRAVENTRICULAR-INJECTION OF AN ANTI-PURKINJE CELL ANTIBODY (ANTI-YO) IN A GUINEA-PIG MODEL
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DOI：
10.1016/0022-510x(91)90198-g
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发表时间：
1991-11-01
影响因子：
4.4
通讯作者：
JUAREZ, C
中科院分区：
文献类型：
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作者：
GRAUS, F;ILLA, I;JUAREZ, C
雌性豚鼠接受脑室注射来自副肿瘤性小脑变性(PCD)患者的免疫球蛋白和抗浦肯野细胞抗体(抗Yo免疫球蛋白)或对照免疫球蛋白。一次注射对照或抗Yo-Ig G后不同时间处死的豚鼠，2 h后在浦肯野细胞胞浆内即可检测到Ig G免疫反应，并持续至2 4h，而最后一次注射后7 d和30 d处死的豚鼠，在最后一次注射后2 4h处死的豚鼠，浦肯野细胞中均未检测到Ig G。在任何动物身上都没有发现小脑受累的临床或病理证据。这项研究表明，抗Yo抗体本身可能不是PCD浦肯野细胞丢失的原因。
Female guinea pigs had intraventricular injections of either IgG from a patient with paraneoplastic cerebellar degeneration (PCD) and anti-Purkinje cell antibodies (anti-Yo IgG) or control IgG. In animals that received a single injection of control or anti-Yo IgG and were killed at different time intervals, IgG immunoreactivity was present in the cytoplasm of Purkinje cells at 2 h and persisted at 24 h. In guinea pigs injected for 15 days with control or anti-Yo IgG and sacrificed 24 h after the last injection, IgG was detected into the Purkinje cells in both groups, whereas animals killed 7 and 30 days after the last injection had no staining for IgG in the Purkinje cells. Clinical or pathologic evidence of cerebellar involvement was not seen in any of the animals. This study suggests the anti-Yo antibody alone may not be the cause of the Purkinje cell loss in PCD.