Expression of the hormonal FGF co-receptor Klotho beta in the Xenopus laevis model
Expression of the hormonal FGF co-receptor Klotho beta in the Xenopus laevis model
复制标题
非洲爪蟾模型中激素 FGF 共受体 Klotho beta 的表达
DOI：
10.1002/cbin.11059
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发表时间：
2019
影响因子：
3.9
通讯作者：
Tao Qinghua
中科院分区：
文献类型：
--
作者：
Chen Geng;Tao Qinghua
Klotho β (Klb)是一种单通道跨膜蛋白，已被描述为内分泌FGFs（如FGF15/19和FGF21）的辅助受体，可调节成年小鼠多个器官和组织的关键代谢过程。然而，它在早期胚胎发育过程中的功能仍是未知的。本文首次采用RT - PCR和全载原位杂交技术对非洲爪蟾早期发育过程中klbmrna的表达进行了评价。RT - PCR实验表明，klb的表达最初在原肠期晚期被检测到，随后在整个胚胎发育过程中快速增加并持续表达。全安装原位杂交在尾芽期的许多原始器官如肝原基和胰腺芽中检测到特异性表达，提示激素FGF信号可能在前肠发育中起作用。klb的动态和特异性表达模式也表明，非洲爪蟾可以为胚胎发育过程中激素FGF信号在器官发生和代谢调节中的功能提供方便的模型。
Klotho beta (Klb), a single‐pass transmembrane protein, has been described as a co‐receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this paper, we evaluated for the first time the expression ofklbmRNA during early development ofXenopus laevisby RT‐PCR and whole mount in situ hybridization. RT‐PCR experiments showed that the expression ofklbwas initially detected at late gastrula stage followed by a quick increasing and continued expression throughout embryonic development. Whole mount in situ hybridization detected specific expression ofklbin many primordial organs at tailbud stage such as liver primordium and pancreatic buds, implying that the hormonal FGF signaling may play a role in the foregut development. The dynamic and specific expression patterns ofklbalso suggest thatXenopus laeviscan serve a convenient model for the function of the hormonal FGF signaling in organogenesis and metabolism regulation during embryonic development.