Stem cells, neural progenitors, and engineered stem cells.
Stem cells, neural progenitors, and engineered stem cells.
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DOI：
10.1007/978-1-4939-2152-2_19
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Iyer S
中科院分区：
文献类型：
--
作者：
Rao RR;Iyer S
人类多能干细胞(HPSCs)具有在体内形成各种类型细胞的独特潜能。这一潜力提供了产生人类祖细胞和其他分化细胞类型的机会，以了解人类发育并用于特定细胞类型的治疗。同样重要的是设计干细胞及其衍生的祖细胞以模拟特定疾病模型的能力。本章将重点介绍hPSCs来源的人类神经前体细胞(HNPs)的繁殖和特性，特别关注工程hNPs以建立人类神经线粒体疾病的体外疾病模型。我们将讨论培养和鉴定hPSCs和hNPs的方法，以及使用一种新的线粒体转基因技术设计hNPs的方案。
Human pluripotent stem cells (hPSCs) have the unique potential to form every cell type in the body. This potential provides opportunities for generating human progenitors and other differentiated cell types for understanding human development and for use in cell type-specific therapies. Equally important is the ability to engineer stem cells and their derived progenitors to mimic specific disease models. This chapter will focus on the propagation and characterization of human neural progenitors (hNPs) derived from hPSCs with a particular focus on engineering hNPs to generate in vitro disease models for human neuro-mitochondrial disorders. We will discuss the methodologies for culturing and characterizing hPSCs and hNPs; and protocols for engineering hNPs by using a novel mitochondrial transfection technology.