Novel Monoclonal Antibodies for Identification of Multicentric Castleman' s Disease ; Kaposi' s Sarcoma-Associated Herpesvirus-Encoded vMIP-I and vMIP-II.
Novel Monoclonal Antibodies for Identification of Multicentric Castleman' s Disease ; Kaposi' s Sarcoma-Associated Herpesvirus-Encoded vMIP-I and vMIP-II.
复制标题
用于鉴定多中心卡斯尔曼氏病的新型单克隆抗体；
作者：
Nakano;K.;Katano;H.;Tadagaki;K.;Sato;Y.;Ohsaki;E.;Mori;Y.;Yamanishi;K.;and Ueda;K.
最近的研究表明，vMIP-Ⅰ和vMIP-Ⅱ在卡波济肉瘤相关疱疹病毒（KSHV）相关疾病的发病机制中发挥重要作用，由于这些蛋白对血管形成的影响。我们开发了针对KSHV编码的病毒巨噬细胞炎性蛋白-I（vMIP-I）和vMIP-II的单克隆抗体，以研究这些表达谱并揭示KSHV相关疾病的发病机制。抗vMIP-Ⅰ和vMIP-Ⅱ的单克隆抗体在裂解诱导后与KSHV感染的细胞系反应。用12-O-十四烷酰基佛波醇-13-乙酸酯诱导后24小时，直到60小时，在原发性渗出性淋巴瘤细胞系的细胞质中检测到vMIP-I和vMIP-II基因产物。在临床标本中，vMIP-Ⅰ和vMIP-Ⅱ基因产物在多中心Castleman病患者的组织中检测到。另一方面，在卡波西肉瘤的一个子集中仅检测到vMIP-Ⅱ。我们的结论是，这些抗体可能是强大的工具，以阐明KSHV相关疾病的发病机制。
Recent studies have indicated that vMIP-I and vMIP-II play important roles in the pathogenesis of Kaposi's sarcoma-associated herpesvirus (KSHV)-related diseases due to the effects of these proteins on vascularization. We developed monoclonal antibodies against KSHV-encoded viral macrophage inflammatory protein-I (vMIP-I) and vMIP-II to study these expression profiles and reveal the pathogenesis of KSHV-related diseases. The MAbs against vMIP-I and vMIP-II reacted to KSHV-infected cell lines after lytic induction. Both vMIP-I and the vMIP-II gene products were detected 24h post-induction with 12-O-tetradecanoylphorbol-13-acetate until 60h in the cytoplasm of primary effusion lymphoma cell lines. In clinical specimens, both vMIP-I and vMIP-II gene products were detected in the tissues of patients with multicentric Castleman's disease. On the other hand, only vMIP-II was detected in a subset of Kaposi's sarcoma. We concluded that these antibodies might be powerful tools to elucidate the pathogenesis of KSHV-related diseases.