Inhibition of CD146 gene expression via RNA interference reduces in vitro perineural invasion on ACC-M cell
Inhibition of CD146 gene expression via RNA interference reduces in vitro perineural invasion on ACC-M cell
复制标题
通过 RNA 干扰抑制 CD146 基因表达可减少体外对 ACC-M 细胞的神经周围侵袭。
DOI：
10.1111/j.1600-0714.2008.00706.x
复制
发表时间：
2009-02-01
影响因子：
3.3
通讯作者：
Sun, M. Y.
中科院分区：
文献类型：
--
作者：
Chen, W.;Zhang, H. L.;Sun, M. Y.
黑色素瘤细胞粘附分子（MCAM或CD 146）是免疫球蛋白基因超家族的新成员，其过表达可促进恶性肿瘤细胞的侵袭、转移、生长和存活。逆转录-聚合酶链反应，蛋白质印迹，3-采用（4，5-二甲基噻唑-2-基）-2，5-二苯基-2H-溴化四氮唑法、流式细胞仪分析和体外神经侵袭（PNI）实验研究CD 146对细胞生长的影响，ACC细胞周期分布和细胞PNI活性我们已经证明，不同的shRNAs可以特异性地敲低CD 146的转录，导致ACC-M细胞增殖的抑制，以及G（0）/G（1）到S期的停滞。CD 146基因在ACC-M PNI活性中的表达下调，提示CD 146是ACC相关基因，在ACC的发生、发展及PNI活性中起重要作用。
Overexpression of melanoma cell adhesion molecule (MCAM or CD146), a novel member of the immunoglobulin gene superfamily, promotes invasion, metastasis, growth and survival of malignant cells.Here, we used a human U6 promoter-driven DNA template approach to induce short hairpin RNA (shRNA)-triggered RNA interference to block CD146 gene expression in the human high-metastatic adenoid cystic carcinoma (ACC) cell line ACC-M. reverse transcription-polymerase chain reaction, Western blot, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, flow cytometry analysis and in vitro perineural invasion (PNI) assay were used to investigate the effects of CD146 on cell growth, cell cycle distribution and cell PNI activity in ACC-M cells.We have demonstrated that different shRNAs can specifically knockdown the transcription of CD146, resulting in the inhibition of cell proliferation, and G(0)/G(1) to S phase arrest in ACC-M cells. Knockdown of CD146 by shRNA resulted in decrease of the ACC-M PNI activity in vitro.These findings suggested that CD146 was an ACC-related gene and CD146 may play an important role in the carcinogenesis, progression and PNI activity of ACC.