HUMAN THY-1 - EXPRESSION ON THE CELL-SURFACE OF NEURONAL AND GLIAL-CELLS
HUMAN THY-1 - EXPRESSION ON THE CELL-SURFACE OF NEURONAL AND GLIAL-CELLS
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DOI：
10.1016/0006-8993(82)90727-2
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发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
GREAVES, MF
中科院分区：
文献类型：
--
作者：
KEMSHEAD, JT;RITTER, MA;GREAVES, MF
本文测定了Thy-1抗原(一种神经组织细胞表面糖蛋白)在4个人神经细胞系和2个神经胶质细胞系以及新鲜的神经源性肿瘤细胞上的分布和数量。在4个神经细胞系中，有3个细胞系(LAN-1、TR14、CHP 212)和GT中90%的细胞为Thy-1+，但LAN-1细胞仅有微弱的免疫荧光，平均每个细胞的Thy-1分子数是TR14或CHP 212的2.4倍。平均每个TR14细胞的Thy-1分子数为.apprx。2.25次。105.在第4代神经元细胞系(CHP 100)中，只有66%的细胞Thy-1+，尽管这些细胞显示出强烈的免疫荧光。两种神经胶质细胞系UCH-203和H314/123在90%的细胞上显示出较强的Thy-1免疫荧光。新鲜的神经元肿瘤细胞，尽管.apprx。80%的肿瘤为Thy-1+(基本上100%的细胞为阳性)，不同肿瘤之间的免疫荧光标记强度有相当大的差异。细胞系的这种异质性和恶性可能反映了体内正常的变异。因此，不同的表型可能代表不同的神经细胞谱系，或者只是同一谱系内成熟状态的差异。正常骨髓中Thy-1+细胞的比例很低(占有核细胞的0.1%)，提示抗Thy-1抗体在神经母细胞瘤的诊断和后续治疗中可能有价值。
The distribution and amount of the Thy-1 antigen (a neural tissue cell surface glycoprotein) was measured on 4 human neuronal and 2 neuroglial cell lines and on fresh tumor cells of neuronal origin. In 3 of 4 neuronal lines (LAN-1, TR14, CHP 212) > 90% of cells were Thy-1+; however, LAN-1 cells showed only weak immunofluorescence and bore on average 2.4 times fewer molecules of Thy-1 per cell than those of TR14 or CHP 212. The mean number of Thy-1 molecules per TR14 cell was .apprx. 2.25 .times. 105. Only 66% of cells in the 4th neuronal line (CHP 100) were Thy-1+, although these showed strong immunofluorescence. Both glial cell lines, UCH-203 and H314/123, showed strong Thy-1 immunofluorescence on > 90% of cells. With fresh neuronal tumor cells, although .apprx. 80% of tumors were Thy-1+ (essentially 100% of cells in these being positive), there were considerable differences in the intensity of labeling by immunofluorescence between different tumors. Such heterogeneity in cell lines and malignancy may reflect normal in vivo variation. Different phenotypes might therefore represent separate neural cell lineages, or simply differences in maturational status within a lineage. The very low frequency of Thy-1+ cells in normal bone marrow (< 0.1% of nucleated cells) indicates that anti-Thy-1 antibodies may be valuable in the diagnosis and subsequent treatment of neuroblastoma.