ISOLATION AND CHARACTERIZATION OF 5 NEW SOLUBLE PLACENTAL TISSUE PROTEINS (PP22, PP23, PP24, PP25, PP26)
ISOLATION AND CHARACTERIZATION OF 5 NEW SOLUBLE PLACENTAL TISSUE PROTEINS (PP22, PP23, PP24, PP25, PP26)
复制标题
DOI：
10.1007/bf02390087
复制
发表时间：
1991-01-01
影响因子：
2.6
通讯作者：
WINCKLER, W
中科院分区：
文献类型：
--
作者：
BOHN, H;WINCKLER, W
从人足月胎盘生理盐水提取液中分离纯化了5种新的可溶性胎盘组织蛋白(pp22、pp23、pp24、pp25、pp26)，并对其理化性质进行了鉴定。用相应的纯化蛋白免疫动物，获得了新蛋白的特异性抗血清。他们被用来通过免疫化学方法检测和定量胎盘和其他人体组织提取物中的新蛋白质，如凝胶扩散试验。新蛋白的免疫组织化学定位以及用灵敏的放射免疫分析方法测量它们在体液中的浓度目前正在研究中。
Five new soluble placental tissue proteins (PP22, PP23, PP24, PP25, PP26) were isolated to purity from saline extracts of human term placentas and characterized by their physico-chemical properties. Specific antisera to the new proteins were obtained by immunizing animals with the corresponding purified proteins. They were used to detect and quantitate the new proteins in extracts of placentas and other human tissues by immunochemical methods such as gel diffusion tests. The immunhistochemical localization of the new proteins as well as measurement of their concentrations in body fluids by sensitive radioimmunoassays are presently under investigation.