Survey of Rice Proteins Interacting With OsFCA and OsFY Proteins Which Are Homologous to the Arabidopsis Flowering Time Proteins, FCA and FY
Survey of Rice Proteins Interacting With OsFCA and OsFY Proteins Which Are Homologous to the Arabidopsis Flowering Time Proteins, FCA and FY
复制标题
DOI：
10.1093/pcp/pcp093
复制
发表时间：
2009-08-01
影响因子：
4.9
通讯作者：
Kim, Jeong-Kook
中科院分区：
文献类型：
--
作者：
Jang, Yun Hee;Park, Hyo-Young;Kim, Jeong-Kook
FCA蛋白参与控制开花时间，并在拟南芥RNA介导的染色质沉默中发挥更普遍的作用。它包含两个RNA结合结构域和一个WW结构域。FCA蛋白通过WW结构域与FY（一种多聚腺苷酸化因子）相互作用。我们以前的特点是水稻基因，OsFCA，这是同源的FCA。在此，我们发现OsFCA蛋白质可以通过其WW结构域与以下蛋白质相互作用：OsFY，一种包含存在于RNA加工因子如Pcf 11和Nrd 1中的CID结构域的蛋白质;一种类似于剪接因子SF 1的蛋白质;一种类似于FUSE剪接因子的蛋白质;和OsMADS 8。FY蛋白与拟南芥中的3端加工机制相关。因此，我们研究了OsFY与AtCstF-50、-64和-77蛋白的水稻同源物（OsCstF-50、-64和-77）之间的相互作用。我们发现OsFY可以结合OsCstF 50，而OsCstF 77蛋白可以桥接OsCstF 50和OsCstF 64之间的相互作用。综上所述，我们的数据表明，OsFCA可以通过其WW结构域与OsFY以外的几种蛋白质相互作用，并可能在水稻中发挥多种作用。
The FCA protein is involved in controlling flowering time and plays more general roles in RNA-mediated chromatin silencing in Arabidopsis. It contains two RNA-binding domains and a WW domain. The FCA protein interacts with FY, a polyadenylation factor, via its WW domain. We previously characterized a rice gene, OsFCA, which was homologous to FCA. Here, we found that the OsFCA protein could interact through its WW domain with the following proteins: OsFY, a protein containing a CID domain present in RNA-processing factors such as Pcf11 and Nrd1; a protein similar to splicing factor SF1; a protein similar to FUSE splicing factor; and OsMADS8. The FY protein is associated with the 3 end processing machinery in Arabidopsis. Thus, we examined interactions between OsFY and the rice homologs (OsCstF-50, -64 and -77) of the AtCstF-50, -64 and -77 proteins. We found that OsFY could bind OsCstF50, whereas the OsCstF77 protein could bridge the interaction between OsCstF50 and OsCstF64. Taken together, our data suggest that OsFCA could interact with several proteins other than OsFY through its WW domain and may play several roles in rice.