Near-Infrared Afterglow Luminescence of Chlorin Nanoparticles for Ultrasensitive In Vivo Imaging
Near-Infrared Afterglow Luminescence of Chlorin Nanoparticles for Ultrasensitive In Vivo Imaging
复制标题
DOI：
10.1021/jacs.1c10168
复制
发表时间：
2022-04-20
影响因子：
15
通讯作者：
Gao, Mingyuan
中科院分区：
文献类型：
--
作者：
Chen, Wan;Zhang, Yuan;Gao, Mingyuan
余辉成像在超灵敏生物医学成像中具有巨大的潜力。由于它在实时光激发停止后检测光子，因此可以有效地消除自发荧光。然而，由于缺乏具有令人满意的寿命、生物相容性和高发光亮度的余辉剂，尤其是用于体内应用的近红外区域的余辉，余辉成像仍然处于婴儿阶段。为了解决这些问题，本研究首次报道了二氢卟酚纳米颗粒（Ch-NPs）发射余辉发光峰值在680 nm处，半衰期长达1.5 h，比其他报道的有机余辉探针长近1个数量级。深入的实验和理论研究表明，余辉发光的亮度与单线态氧（O-1（2））的容量和二氢卟酚的氧化性密切相关。得益于超长的半衰期和最小化的成像背景，在注射前单次激发产生的余辉发光的引导下，成功切除了3 mm（3）的小转移性肿瘤病灶，由于组织自体荧光，这对于传统的近红外荧光成像是不可能的。
Afterglow imaging holds great potential for ultrasensitive biomedical imaging. As it detects photons after the cessation of real-time light excitation, autofluorescence can therefore be effectively eliminated. However, afterglow imaging is still in its infant stage due to the lack of afterglow agents with satisfactory lifetime, biocompatibility, and high luminescence brightness, particularly afterglow in the near-infrared region for in vivo applications. To address these issues, this study for the first time reports chlorin nanoparticles (Ch-NPs) emitting afterglow luminescence peaking at 680 nm with a half-life of up to 1.5 h, which is almost 1 order of magnitude longer than those of other reported organic afterglow probes. In-depth experimental and theoretical studies revealed that the brightness of the afterglow luminescence is strongly correlated with the singlet oxygen (O-1(2)) capacity and the oxidizability of the chlorins. Benefitting from the ultralong half-life and the minimized imaging background, small metastatic tumor foci of 3 mm(3) were successfully resected under the guidance of the afterglow luminescence generated upon a single shot of activation prior to the injection, which was impossible for conventional near-infrared fluorescence imaging due to tissue autofluorescence.