Using natural 13C abundances to differentiate between three CO2 sources during incubation of a grassland soil amended with slurry and sugar
Using natural 13C abundances to differentiate between three CO2 sources during incubation of a grassland soil amended with slurry and sugar
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DOI：
10.1002/jpln.200421412
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发表时间：
2004-12-01
期刊：
影响因子：
--
通讯作者：
Bol, R
中科院分区：
文献类型：
--
作者：
Kuzyakov, Y;Bol, R
这项研究描述了一种新的方法，用一种示踪法(这里是C-13自然丰度)来分离三种土壤碳(C)源。该方法利用添加C-3或C-4泥浆和/或C-3或C-4糖改良的C-3草原土壤的CO2外流的时间动态来估计三种不同的碳源(天然土壤、泥浆和糖)对CO2外流的贡献。含有泥浆和/或糖的土壤在受控条件下培养，并在两周内测量释放出的二氧化碳的浓度和增量(13)C值。研究并证明了在CO2排放中分离三种碳源所需的主要假设，即土壤中C-3和C-4糖的相同分解。对二氧化碳外流的相对贡献较高，但随着碳源(微生物)可利用性的增加，即糖、浆液和SOM的增加，相对贡献较小。文中还讨论了该方法的缺点和局限性以及未来可能的应用。
This study describes a novel approach to separate three soil carbon (C) sources by one tracer method (here C-13 natural abundance). The approach uses the temporal dynamics of the CO2 efflux from a C-3 grassland soil amended with added C-3 or C-4 slurry and/or C-3 or C-4 sugar to estimate contributions of three separate C sources (native soil, slurry, and sugar) to CO2 efflux. Soil with slurry and/or sugar was incubated under controlled conditions, and concentration and delta(13)C values of evolved CO2 were measured over a 2-week period. The main assumption needed for separation of three C sources in CO2 efflux, i.e., identical decomposition of applied C-3 and C-4 sugars in soil, was investigated and proven. The relative contribution to the CO2 efflux was higher, but shorter with an increased (microbial) availability of the C source, i.e., sugar > slurry > SOM. The shortcomings and limitations as well as possible future applications of the suggested method are discussed.