A survey of flagellate diversity at four deep-sea hydrothermal vents in the Eastern Pacific Ocean using structural and molecular approaches
A survey of flagellate diversity at four deep-sea hydrothermal vents in the Eastern Pacific Ocean using structural and molecular approaches
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DOI：
10.1111/j.1550-7408.2000.tb00067.x
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发表时间：
2000-07-01
影响因子：
2.2
通讯作者：
Anderson, OR
中科院分区：
文献类型：
--
作者：
Atkins, MS;Teske, AP;Anderson, OR
在4个深海热液喷口(Juan de Fuca Ridge(2,200 m)、Guayma盆地(2,000 m)、北纬21度(2,550 m)和北纬9度(2,000 m))前分离培养了9个种的18株鞭毛原生动物。光镜和电子显微镜被用来识别鞭毛虫属，并在可能的情况下识别种。对来自浅水区和美国典型养殖库的每一个喷口物种和相关菌株的核糖体小亚基RNA基因进行测序，并与数据库序列进行比较分析，将分类单元放置在rDNA树中。热液喷口鞭毛虫分属于六个不同的分类目：珊瑚藻目、双囊藻目、尾鞭目、鞭毛目、叶甲目和动质目。对Vent分离株和数据库序列的比较分析解决了一些先前分类亲缘关系不确定的知名物种的系统放置问题，如Ancyromonas sigmoidas、Caecitellus parverus和Mussisteria marina。其中许多喷口分离株是世界各地海洋、淡水和陆地生态系统中无处不在的成员，这表明这些鞭毛虫物种在全球范围内分布。
Eighteen strains of flagellated protists representing nine species were isolated and cultured front four deep-sea hydrothermal vents: Juan de Fuca Ridge (2,200 m), Guaymas Basin (2,000 m), 21 degrees N (2,550 m) and 9 degrees N (2,000 m). Light and electron microscopy were used to identify flagellates to genus and, when possible, species. The small subunit ribosomal RNA genes of each vent species and related strains from shallow-waters and the American Type Culture Collection were sequenced then used for comparative analysis with database sequences to place taxa in an rDNA tree. The hydrothermal vent flagellates belonged to six different taxonomic orders: the Ancyromonadida, Bicosoecida, Cercomonadida, Choanoflagellida, Chrysomonadida, and Kinetoplastida. Comparative analysis of vent isolate and database sequences resolved systematic placement of some well-known species with previously uncertain taxonomic affinities, such as Ancyromonas sigmoides, Caecitellus parvulus, and Mussisteria marina. Many of these vent isolates are ubiquitous members of marine, freshwater, and terrestrial ecosystems worldwide, suggesting a global distribution of these flagellate species.