Forced swim test-induced endocrine and immune changes in the rat: Effect of subacute desipramine treatment
Forced swim test-induced endocrine and immune changes in the rat: Effect of subacute desipramine treatment
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DOI：
10.1016/s0091-3057(97)00316-x
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发表时间：
1998-01-01
影响因子：
3.6
通讯作者：
Leonard, BE
中科院分区：
文献类型：
--
作者：
Connor, TJ;Kelly, JP;Leonard, BE
以前有报道称，强迫游泳试验（FST）暴露激活HPA轴，并产生大鼠细胞和非细胞免疫的改变。此外，有证据表明，抗抑郁药预处理对FST诱导的免疫变化具有保护作用。本研究的目的是检查亚急性治疗与三环类抗抑郁药地昔帕明（10毫克/公斤，5和10毫克/公斤; IP）在FST不动的影响，和FST诱导的内分泌和免疫参数的变化在大鼠。以10 mg/kg剂量的维生素B1治疗在FST中产生不动时间的显著减少，而5 mg/kg剂量无效。FST暴露产生了显着增加血清皮质酮和肾上腺抗坏血酸浓度的下降，这两个都没有显着衰减的预处理。有一个轻微的，但不显着的抑制PHA诱导的淋巴细胞增殖15分钟后FST曝光。然而，在该时间点，顺铂处理产生了淋巴细胞增殖的显著增加。FST暴露引起外周血中淋巴细胞百分比的减少和中性粒细胞百分比的增加;抗氧化剂治疗未能显着改变这些应激诱导的变化。FST暴露后120 min，在DMI处理动物中观察到相对脾脏重量显著降低，并伴有循环RBC浓度升高。总之，尽管FST诱导的行为变化通过ESTA处理正常化，但FST暴露诱导的外周畸变（淋巴细胞增殖除外）未正常化。此外，在对照动物中，地塞米松预处理诱导皮质酮、肾上腺抗坏血酸和白细胞亚群的应激样变化。(C)1998年爱思唯尔科学公司
Previously it has been reported that forced swim test (FST) exposure activates the HPA-axis and produces alterations in both cellular and noncellular immunity in rats. Furthermore, there is evidence to suggest that pretreatment with antidepressants has a protective effect against FST-induced immune changes. The purpose of the present study was to examine the effect of subacute treatment with the tricyclic antidepressant desipramine (DMI, 5 and 10 mg/kg; IP) on immobility in the FST, and on FST-induced changes in endocrine and immune parameters in the rat. DMI treatment at a dose of 10 mg/kg produced a significant reduction in immobility time in the FST, while the 5 mg/kg dose was ineffective. FST exposure produced a significant increase in serum corticosterone and a decrease in adrenal ascorbic acid concentrations, neither of which were significantly attenuated by DMI pretreatment. There was a slight but nonsignificant suppression of PHA-induced lymphocyte proliferation 15 min post-FST exposure. However, DMI treatment produced a significant increase in lymphocyte proliferation at this time point. FST exposure caused a reduction in the percentage of lymphocytes and an increase in the percentage of neutrophils in the peripheral blood; DMI treatment failed to significantly alter these stress-induced changes. There was a profound reduction in relative spleen weight observed in DMI-treated animals 120 min post-FST exposure and this was accompanied by an increase in circulating RBC concentrations. In conclusion, although the FST-induced behavioral changes were normalized by DMI treatment the peripheral aberrations induced by FST exposure (with the exception of lymphocyte proliferation) were not. In addition, DMI pretreatment induced stress-like changes in corticosterone, adrenal ascorbic acid and leucocyte subpopulations in the control animals. (C) 1998 Elsevier Science Inc.