A Keratin Biomaterial Gel Hemostat Derived from Human Hair: Evaluation in a Rabbit Model of Lethal Liver Injury
A Keratin Biomaterial Gel Hemostat Derived from Human Hair: Evaluation in a Rabbit Model of Lethal Liver Injury
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DOI：
10.1002/jbm.b.31251
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发表时间：
2009-07-01
影响因子：
3.4
通讯作者：
Van Dyke, Mark
中科院分区：
文献类型：
--
作者：
Aboushwareb, Tamer;Eberli, Daniel;Van Dyke, Mark
需要与组织相容的有效止血敷料。角蛋白是一类生物材料，可以从人类头发中提取蛋白质。我们最近发现角蛋白生物材料具有止血特性，并假设具有吸收液体和结合细胞能力的角蛋白水凝胶可能是一种有效的止血剂。本研究的目的是测试角蛋白水凝胶，并将其与目前的止血剂进行比较。32只新西兰大白兔接受了致命的肝损伤。8只动物分别被分配到阴性对照组、QuickClotu (R)组、HemCon (R)绷带组和角蛋白治疗组。术中记录生命体征等数据，72 h后处死所有存活动物。对所有存活动物进行组织学检查。阴性对照组、QuickClot组、HemCon组和角蛋白组24小时生存率分别为0%、62.5%、62.5%、抗75%。其他结果包括失血、平均动脉压、心率、休克指数和肝脏组织学。术后各时间点止血效果均优于阴性对照组。角蛋白组的止血效果与商用止血药一样好，甚至更好。组织学显示角蛋白组在止血剂-肝脏界面处有有趣的愈合反应。(C) 2008 Wiley期刊有限公司[J]中国生物医学工程学报，2009,31 (4):559 - 564
Effective hemostatic dressings that are compatible with tissues are needed. Keratins are a class of biomaterials that can be derived by extraction of proteins from human hair. We have recently discovered that keratin biomaterials have hemostatic characteristics and hypothesize that a keratin hydrogel having the ability to absorb fluid and bind cells may be an effective hemostat. The goal of this study was to test a keratin hydrogel and evaluate it compared to current hemostats. Thirty-two New Zealand white rabbits received a lethal liver injury. Eight animals each were assigned to negative control, QuickClotu (R), HemCon (R) bandage, and keratin treatment groups. Vital stats and other data were recorded during surgery and all surviving animals were sacrificed after 72 h. Histology was conducted on all surviving animals. Twenty-four-hour survival rates were 0%, 62.5%, 62.5%, anti 75% for the negative control, QuickClot, HemCon, and keratin groups, respectively. Other outcomes included blood loss, mean arterial pressure, heart rate, shock index, and liver histology. All of the hemostats were statistically better than the negative control group at late operative time points. The keratin group consistently performed as well as, or better than, the commercial hemostats. Histology showed an interesting healing response at the hemostat-liver interface in the keratin group. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Apt Biomater 90B: 45-54, 2009