An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry.
An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry.
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DOI：
10.1371/journal.pone.0175415
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Olsen RE
中科院分区：
文献类型：
--
作者：
Betancor MB;Li K;Sprague M;Bardal T;Sayanova O;Usher S;Han L;Måsøval K;Torrissen O;Napier JA;Tocher DR;Olsen RE
需要新的欧米茄3（n-3）长链多不饱和脂肪酸（LC-PUFA）的从头来源作为水产饲料中鱼油的替代品，以保持足够水平的有益脂肪酸，二十碳五烯酸和二十二碳六烯酸（分别为EPA和DHA）。本研究调查了在含有低水平鱼粉（35%）和鱼油（10%）的大西洋鲑鱼（萨尔莫salar）饲料中使用源自转基因亚麻荠的EPA+DHA油，以确定对养殖鲑鱼的组织脂肪酸谱、肠道转录组和健康的影响。用三种实验饮食中的一种（含有鱼油/菜籽油（FO）、野生型亚麻荠油（WCO）或转基因亚麻荠油（DCO）的共混物作为添加的脂质源）喂养二龄鲑后大西洋鲑鱼12周。DCO饮食不影响任何鱼的性能或健康参数的研究。中肠和后肠转录组的分析表明，对代谢只有轻微的影响。喂食DCO饮食的鱼的肉积累的n-3 LC-PUFA的量几乎是喂食FO或WCO饮食的鱼的两倍，这表明来自转基因油籽的这些油提供了将养殖鱼中的n-3 LC-PUFA增加到与十年前发现的水平相当的机会。
New de novo sources of omega 3 (n-3) long chain polyunsaturated fatty acids (LC-PUFA) are required as alternatives to fish oil in aquafeeds in order to maintain adequate levels of the beneficial fatty acids, eicosapentaenoic and docosahexaenoic (EPA and DHA, respectively). The present study investigated the use of an EPA+DHA oil derived from transgenic Camelina sativa in Atlantic salmon (Salmo salar) feeds containing low levels of fishmeal (35%) and fish oil (10%), reflecting current commercial formulations, to determine the impacts on tissue fatty acid profile, intestinal transcriptome, and health of farmed salmon. Post-smolt Atlantic salmon were fed for 12-weeks with one of three experimental diets containing either a blend of fish oil/rapeseed oil (FO), wild-type camelina oil (WCO) or transgenic camelina oil (DCO) as added lipid source. The DCO diet did not affect any of the fish performance or health parameters studied. Analyses of the mid and hindgut transcriptomes showed only mild effects on metabolism. Flesh of fish fed the DCO diet accumulated almost double the amount of n-3 LC-PUFA than fish fed the FO or WCO diets, indicating that these oils from transgenic oilseeds offer the opportunity to increase the n-3 LC-PUFA in farmed fish to levels comparable to those found a decade ago.