Identification of Stem-Like Cells in Atrial Myxoma by Markers CD44, CD19, and CD45.
Identification of Stem-Like Cells in Atrial Myxoma by Markers CD44, CD19, and CD45.
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通过标记 CD44、CD19 和 CD45 鉴定心房粘液瘤中的干细胞样细胞。
DOI：
10.1155/2016/2059584
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发表时间：
2016
影响因子：
4.3
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Song X;Liu D;Cui J;Zhou M;Wang H;Liu N;Qi X;Li Z
心房粘液瘤是最常见的肿瘤，主要发生在房间隔，其起源尚不清楚。据报道，干细胞样细胞亚群存在于良性肿瘤中，并负责肿瘤的发生和维持。在这项研究中，我们调查了干细胞样细胞是否可以有助于心房心脏粘液瘤。免疫组织学数据证实，一个人口的细胞轴承的表面标志物CD19，CD45和CD44居住在粘液瘤的粘多糖丰富的基质。此外，我们用相亮培养法分离粘液瘤细胞，并证实粘液瘤衍生的细胞表达稳健水平的CD 19、CD 45和CD 44。此外，还通过体外心肌细胞和平滑肌细胞分化验证了该细胞群体的多能性。我们的研究结果表明，原发性心脏粘液瘤可能源于间充质干细胞，具有产生肿瘤的多系分化能力。总之，本研究首次证实了干细胞样细胞存在于心房粘液瘤中，并且该细胞群可能具有粘液瘤起始和进展的能力。
Atrial myxoma is the most frequent tumor arising mainly in atrial septum and its origin remains uncertain. It has been reported that a subpopulation of stem-like cells are present in benign tumors and responsible for tumor initiation and maintenance. In this study, we investigated whether stem-like cells could contribute to the atrial cardiac myxoma. Immunohistology data confirmed that a population of cells bearing the surface markers CD19, CD45, and CD44 resided in a mucopolysaccharide-rich matrix of myxoma. Moreover, we isolated myxoma cells with phase-bright culture method and confirmed that myxoma derived cells express robust level of CD19, CD45, and CD44. Furthermore, the pluripotency of this population of cells also was validated by cardiomyocytes and smooth muscle cells differentiation in vitro. Our results indicate that primary cardiac myxoma may arise from mesenchymal stem cells with the ability to generate tumors with multilineage differentiation. In conclusion, this study for the first time verified that stem-like cells are present in atrial myxoma and this population of cells may have the capacity for myxoma initiation and progression.