Rapid Detection of Pseudomonas aeruginosa Biofilms via Enzymatic Liquefaction of Respiratory Samples
Rapid Detection of Pseudomonas aeruginosa Biofilms via Enzymatic Liquefaction of Respiratory Samples
复制标题
DOI：
10.1021/acssensors.0c01618
复制
发表时间：
2020-12-24
期刊：
影响因子：
8.9
通讯作者：
de la Rica, Roberto
中科院分区：
文献类型：
--
作者：
Clemente, Antonio;Alba-Patino, Alejandra;de la Rica, Roberto
由多重耐药的铜绿假单胞菌引起的呼吸道感染，如果不能立即发现，往往会产生不良的结果。然而，用快速诊断测试在呼吸道样本中检测这种病原体是具有挑战性的，因为病原体产生的保护性生物膜本身被高粘度的痰基质包围。在这里，我们介绍了一种在一分钟内液化呼吸道样本并现场破坏细菌生物膜的方法。它依赖于细菌过氧化氢酶通过添加过氧化氢来产生氧气泡。当与纸制移动生物传感器相结合时，所产生的诊断试剂盒能够在8分钟内以极高的灵敏度和特异度检测患者痰中的铜绿假单胞菌感染。快速的周转时间和很少的基础设施要求使该方法成为在护理点快速筛查铜绿假单胞菌感染的理想方法。
Respiratory infections caused by multi-drug-resistant Pseudomonas aeragmosa often yield poor outcomes if not detected right away. However, detecting this pathogen in respiratory samples with a rapid diagnostic test is challenging because the protective biofilms created by the pathogen are themselves surrounded by a high-viscosity sputum matrix. Here, we introduce a method for liquefying respiratory samples and disrupting bacterial biofilms on the spot within a minute. It relies on the generation of oxygen bubbles by bacterial catalase through the addition of hydrogen peroxide. When coupled with a mobile biosensor made of paper, the resulting diagnostic kit was able to detect P. aeruginosa infections in sputa from patients with excellent sensitivity and specificity within 8 min. The quick turnaround time along with few infrastructure requirements make this method ideal for the rapid screening of P. aeruginosa infections at the point of care.