Improving the Yield of Blood Cultures from Patients with Early Lyme Disease
Improving the Yield of Blood Cultures from Patients with Early Lyme Disease
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DOI：
10.1128/jcm.00350-11
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发表时间：
2011-06-01
影响因子：
9.4
通讯作者：
Wormser, Gary P.
中科院分区：
文献类型：
--
作者：
Liveris, Dionysios;Schwartz, Ira;Wormser, Gary P.
根据巴伯-斯托纳-凯利培养基中伯氏疏螺旋体的显微镜检测，大约45%未经治疗的美国早期莱姆病伴移行性红斑患者在孵育2至12周后血液培养呈阳性。在这项研究中，我们证明，血培养的产量可以显着增加到70.8%，通过使用联合培养-定量PCR技术，并在这些患者中发现有一个阳性的血培养，阳性检测在超过90%的孵育7天内。多发性游走性红斑患者几乎一致培养阳性，通过这种技术。
Approximately 45% of untreated United States patients with early Lyme disease associated with erythema migrans have a positive blood culture based on microscopic detection of Borrelia burgdorferi in Barbour-Stoenner-Kelly medium after 2 to 12 weeks of incubation. In this study we demonstrate that the yield of blood cultures can be significantly increased to 70.8% by the use of a combined culture-quantitative PCR technique and that among those patients found to have a positive blood culture, positivity was detected in over 90% within just 7 days of incubation. Patients with multiple erythema migrans were almost uniformly culture positive by this technique.