Polarity of T cell shape, motility, and sensitivity to antigen
Polarity of T cell shape, motility, and sensitivity to antigen
复制标题
DOI：
10.1016/s1074-7613(00)80409-4
复制
发表时间：
1996-05-01
期刊：
影响因子：
32.4
通讯作者：
Cahalan, MD
中科院分区：
文献类型：
--
作者：
Negulescu, PA;Krasieva, TB;Cahalan, MD
T细胞活化需要与APC接触。我们使用光学技术来证明T细胞极性的基础上的形状，运动性和局部抗原的敏感性。细胞内Ca 2+钳夹显示T细胞的形状和运动性对[Ca 2 +]（i）（Kd = 200 nM）的变化极其敏感，通过钙调神经磷酸酶非依赖性途径发生固定和变圆。Ca 2+依赖性固定延长了T细胞与抗原呈递B细胞的接触;缓冲[Ca 2 +]（i）信号阻止了稳定细胞对的形成。光镊通过控制B细胞或α-CD 3 MAb包被的珠粒在T细胞表面上的放置，揭示了空间T细胞对抗原的敏感性。与尾部相比，T细胞对在T细胞前缘处进行的接触的敏感性高4倍。我们的结论是，运动T细胞是极化的抗原传感器，物理响应[Ca 2 +]（i）信号，以稳定其与APC的相互作用。
T cell activation requires contact with APCs. We used optical techniques to demonstrate T cell polarity on the basis of shape, motility, and localized sensitivity to antigen. An intracellular Ca2+ clamp showed that T cell shape and motility are extremely sensitive to changes in [Ca2+](i) (K-d = 200 nM), with immobilization and rounding occurring via a calcineurin-independent pathway. Ca2+-dependent immobilization prolonged T cell contact with the antigen-presenting B cell; buffering the [Ca2+](i) signal prevented the formation of stable cell pairs. Optical tweezers revealed spatial T cell sensitivity to antigen by controlling placement on the T cell surface of either B cells or alpha-CD3 MAb-coated beads. T cells were 4-fold more sensitive to contact made at the leading edge of the T cell compared with the tail. We conclude that motile T cells are polarized antigen sensors that respond physically to [Ca2+](i) signals to stabilize their interaction with APCs.