Comprehensive transcriptome analysis reveals common and specific genes and pathways involved in cold acclimation and cold stress in tea plant leaves
Comprehensive transcriptome analysis reveals common and specific genes and pathways involved in cold acclimation and cold stress in tea plant leaves
复制标题
综合转录组分析揭示了茶树叶片冷驯化和冷应激中常见和特异的基因和途径
DOI：
10.1016/j.scienta.2018.06.008
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发表时间：
2018-10-20
影响因子：
4.3
通讯作者：
Wang, Xinchao
中科院分区：
文献类型：
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作者：
Hao, Xinyuan;Wang, Bo;Wang, Xinchao
冬季的冰冻温度以及冬季和早春的异常温度波动是威胁茶树越冬的最有害的环境因素，并可能导致春季茶叶生产的显着经济损失。本研究模拟自然气候变化，建立冷驯化和快速冷胁迫条件，研究冷驯化和冷胁迫中转录组的变化。结果表明，在CA的初始阶段发生的转录变化和细胞壁的变化，发生在整个CA过程中，这些变化在提高抗冻性在这个过程中发挥了至关重要的作用。低温胁迫下，不同的低温应答机制被迅速激活，但随后的低温诱导活性氧的积累可能是刺激胁迫相关基因表达、损害叶片生理的主要信号和有害因素。此外，我们研究了60个差异表达的基因共享的两个过程，并强调了β-酮脂酰CoA脱氢酶，HXXXD型酰基转移酶家族蛋白，NAC域包含蛋白80，糖SWEET转运蛋白和烯醇化酶的重要性，在各种寒冷条件下的反应。研究结果为了解茶树对复杂低温条件的响应机制提供了有用的信息。
Freezing temperatures during the winter and unusual temperature fluctuations during the winter and early spring are the most harmful ambient factors threatening tea plant winter survival and may cause marked economic losses in tea production during the spring. In this study, we simulate natural climate change, to established cold acclimation (CA) and rapid cold stress conditions to investigate the transcriptome changes involved in CA and cold stress. Results revealed transcriptional changes occurring during the initial period of CA and the cell wall changes that occur throughout the entire CA process; these changes play crucial roles in increasing freezing tolerance during this process. Comparing cold-acclimated plants without further treatment against cold-acclimated plants under cold-stress, different cold response mechanisms were rapidly activated under cold stress; however, the subsequent freezing-induced accumulation of reactive oxygen species could be the major signal and harmful factor stimulating stress-associated gene expression and impairing tea leaf physiology. Moreover, we investigated 60 differentially expressed genes shared by both processes and highlighted the importance of beta-ketoacyl CoA synthases, HXXXD-type acyl-transferase family proteins, NAC domain containing protein 80, sugar SWEET transporters and enolases in the responses to various cold conditions. The results provide useful information for understanding the regulation mechanism in tea plant responding to complex low temperature conditions.