Origins of the enhanced affinity of RNA-protein interactions triggered by RNA phosphorodithioate backbone modification.
Origins of the enhanced affinity of RNA-protein interactions triggered by RNA phosphorodithioate backbone modification.
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RNA 二硫代磷酸酯主链修饰引发的 RNA-蛋白质相互作用亲和力增强的起源。
DOI：
10.1039/c7cc05722a
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Egli,Martin
中科院分区：
文献类型：
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作者：
Yang,Xianbin;DinukaAbeydeera,N;Liu,Feng-Wu;Egli,Martin
利用生物层干涉法（BLI）分析了MS2外壳蛋白与其同源RNA发夹之间的相互作用，以评估硫代磷（PS2）取代磷酸盐对其亲和力的影响。基于结构的数据分析提供了对PS2片段触发的rna -蛋白相互作用增强亲和力的起源的见解。
The well-characterized interaction between the MS2 coat protein and its cognate RNA hairpin was used to evaluate changes in affinity as a result of phosphorodithioate (PS2) replacing phosphate by biolayer interferometry (BLI). A structure-based analysis of the data provides insights into the origins of the enhanced affinity of RNA–protein interactions triggered by the PS2 moiety.