Dendritic cells are the principal cells in mouse spleen bearing immunogenic fragments of foreign proteins.
Dendritic cells are the principal cells in mouse spleen bearing immunogenic fragments of foreign proteins.
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DOI：
10.1084/jem.172.1.383
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发表时间：
1990-07-01
影响因子：
15.3
通讯作者：
Steinman, R M
中科院分区：
文献类型：
--
作者：
Crowley, M;Inaba, K;Steinman, R M
我们监测了从小鼠脾脏和腹膜腔中取出的细胞的 APC 功能，这些小鼠已通过静脉内或腹膜内途径给予蛋白质抗原。使用 mAb 33D1 和 N418 阴性和阳性选择树突状细胞，我们获得的证据表明树突状细胞是脾脏中的主要细胞类型，其携带对抗原特异性 T 细胞具有免疫原性形式的蛋白质。体内脉冲巨噬细胞不具有免疫原性，并且似乎不能将肽片段转移至树突状细胞。
We monitored the APC function of cells taken from the spleen and peritoneal cavity of mice that had been given protein antigens via the intravenous or intraperitoneal routes. Using the mAb 33D1 and N418 to negatively and positively select dendritic cells, we obtained evidence that dendritic cells are the main cell type in spleen that carries the protein in a form that is immunogenic for antigen-specific T cells. In vivo pulsed macrophages were not immunogenic and did not appear capable of transferring peptide fragments to dendritic cells.