Homologous recombination in the Dictyostelium alpha‐actinin gene leads to an altered mRNA and lack of the protein.
Homologous recombination in the Dictyostelium alpha‐actinin gene leads to an altered mRNA and lack of the protein.
复制标题
盘基网柄菌 α-辅肌动蛋白基因中的同源重组导致 mRNA 改变和蛋白质缺失。
作者：
Walter Witke;Wolfgang Nellen;Angelika A. Noegel
通过用含有α-辅肌动蛋白基因的1.2 kb片段的网柄藻转化载体pDNeo II转化细胞，实现了网柄藻中α-辅肌动蛋白基因的突变。缺乏α辅肌动蛋白（一种肌动蛋白结合蛋白）的转化体产生了一种改变的mRNA，该mRNA缺乏编码区的3′部分。α-辅肌动蛋白产生的缺陷不是由于基因内载体的整合，而是显然是由引入的α-辅肌动蛋白序列与内源基因编码区中其互补序列之间同源重组期间产生的错误引起的。
Mutation of the alpha‐actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha‐actinin gene. Transformants deficient in alpha‐actinin, an actin‐binding protein, produced an altered mRNA that lacked the 3′ portion of the coding region. The defect in alpha‐actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha‐actinin sequence and its complementary sequence in the coding region of the endogenous gene.