Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice
Elimination of atretic follicles from the mouse ovary: a TEM and immunohistochemical study in mice
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DOI：
10.1046/j.1469-7580.2000.19610103.x
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发表时间：
2000-01-01
影响因子：
2.4
通讯作者：
Tonosaki, A
中科院分区：
文献类型：
--
作者：
Inoue, S;Watanabe, H;Tonosaki, A
我们通过透射电子显微镜和光学显微镜免疫组织化学检查了 32-35 天处女小鼠的大量卵泡。没有看到巨噬细胞，但遇到了不同阶段的凋亡颗粒细胞。据推测，处于细胞凋亡晚期的颗粒细胞或其碎片注定会被邻近的看起来正常的颗粒细胞吞噬。在与萎缩卵母细胞接触并明显吞噬萎缩卵母细胞的透明带区域中观察到其他外观正常的颗粒细胞。这种颗粒细胞的特征在于与其他细胞存在间隙连接，并且细胞质中经常含有环形间隙连接。为了确认卵泡闭锁和消除过程中缺乏巨噬细胞的参与，将专门制备的卵巢切片与抗小鼠巨噬细胞单克隆抗体（F4/80、Mac-1、Mac-2）一起孵育。检查的卵泡均未显示出与这些抗体的阳性免疫反应性。由于颗粒细胞反复凋亡和吞噬作用，闭锁卵泡可能会缩小并最终从卵巢中消失。没有证据表明闭锁卵泡中存在或参与巨噬细胞？至少在所检查的繁殖前小鼠中是这样。
We examined numerous ovarian follicles from 32-35 d virgin mice by transmission electron microscopy and light microscopic immunohistochemistry. No macrophages were seen, but various stages of apoptotic granulosa cells were encountered. Presumably a granulosa cell or its debris in an advanced stage of apoptosis was destined to be phagocytosed by adjacent normal-looking granulosa cells. Other granulosa cells of normal appearance were seen in the region of the zona pellucida in contact with and apparently phagocytosing atrophic oocytes. Such granulosa cells were characterised by the presence of gap junctions with other cells and frequently contained annular gap junctions in the cytoplasm. To confirm the lack of involvement of macrophages in the process of follicular atresia and elimination, specially prepared ovarian sections were incubated with antimouse macrophage monoclonal antibodies (F4/80, Mac-1, Mac-2). None of the follicles examined showed positive immunoreactivity with these antibodies. Atretic follicles may shrink and eventually disappear from the ovary as a result of repeated apoptosis and phagocytosis by granulosa cells. There is no evidence for the presence or involvement of macrophages in the atretic follicles? at least in prereproductive mice as examined.