Effects of Escherichia coli K12 Biofilm on Sensor Thin Film Materials
Effects of Escherichia coli K12 Biofilm on Sensor Thin Film Materials
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DOI：
10.1109/sensors43011.2019.8956741
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发表时间：
2019-10
期刊：
影响因子：
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通讯作者：
M. McGlennen;Markus Dieser;C. Foreman;S. Warnat
中科院分区：
文献类型：
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作者：
M. McGlennen;Markus Dieser;C. Foreman;S. Warnat
微型传感器能够以低成本研究河流、湖泊或海洋等水环境中的化学和物理动力学。传感器必须在这些环境中可靠地工作，其中包括生物和化学挑战。然而，尚未详细研究传感器薄膜在水性应用中的应用，更具体地说，生物相互作用如何改变传感器材料的特性。在本研究中，研究了生物膜形成对铝（电导体）和 a-SixNy:H（绝缘材料）性能的长期影响。大肠杆菌 K12 生物膜生长引起的材料降解通过 7 周时间内的薄层电阻测量（共线四点探针）和傅里叶变换红外光谱 (FTIR) 吸收光谱来确定。使用扫描电子显微镜（SEM）和白光干涉测量法测试表面形貌的变化。发现铝在三周内严重降解，而 a-SixNy:H 在整个调查期间呈惰性。由于薄膜传感器材料之间的差异很明显，因此应该探索包括更广泛的材料在内的更详细的研究。
Micro-fabricated sensors enable the study of chemical and physical dynamics in aqueous environments such as rivers, lakes or oceans at low cost. Sensors must work reliably in these environments, which include both biological and chemical challenges. However, sensor thin films have not been studied in detail for aqueous applications, and more specifically how biotic interactions may change sensor material properties. In this study, the long-term effects of biofilm formation on the properties of aluminum (electric conductor) and a-SixNy:H (insulating material) were investigated. Material degradation caused by Escherichia coli K12 biofilm growth was determined by electrical sheet resistance measurements (collinear four-point-probe) and Fourier-transform infrared spectroscopy (FTIR) absorption spectra over a time period of 7 weeks. Changes of the surface topography were tested using scanning electron microscopy (SEM) and white light interferometry. Aluminum was found to be heavily degraded at three weeks, whereas a-SixNy:H was inert during the entire investigation period. As differences between thin film sensor materials are evident, more detailed investigations including a broader range of materials should be explored.