INCREASED EXPRESSION OF ADHESION MOLECULES (CD54, CD29 AND CD44) ON FIBROBLASTS INFECTED WITH CYTOMEGALOVIRUS
INCREASED EXPRESSION OF ADHESION MOLECULES (CD54, CD29 AND CD44) ON FIBROBLASTS INFECTED WITH CYTOMEGALOVIRUS
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DOI：
10.1111/j.1348-0421.1995.tb02179.x
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发表时间：
1995-01-01
影响因子：
2.6
通讯作者：
SAKURAI, M
中科院分区：
文献类型：
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作者：
ITO, M;WATANABE, M;SAKURAI, M
用新细胞术分析了细胞间介素-1 （CD54）、β 1整合素（CD29）和CD44在巨细胞病毒（CMV）感染的人胚胎成纤维细胞（HEF）中的表达。与未感染的HEF相比，这些粘附分子在cmv感染的HEF上的表达在接种后第2天和第5天显著增加。然而，这些粘附分子在单纯疱疹病毒(HSV)-1和水痘-带状疱疹病毒（VZV）感染的HEF中表达减少。无论是用灭活的巨细胞病毒还是用感染巨细胞病毒的细胞上清液处理HEF，均未观察到表达增加。添加抗细胞因子（tnf - α、IL-1 β或ifn - γ）抗体对这些粘附分子的增加没有影响。这表明cmv感染细胞上CD54、CD29和CD44的增加需要活跃的病毒复制，而不是由cmv感染细胞释放的可溶性因子介导的。CMV感染的成纤维细胞粘附分子的变化可能有助于CMV感染引起的炎症。
The expression of ICAM-1 (CD54), beta 1 integrin (CD29), and CD44 on cytomegalovirus (CMV)-infected human embryonic fibroblasts (HEF) was analyzed by hew cytometry. The expression of these adhesion molecules increased significantly on CMV-infected HEF, on days 2 and 5 after inoculation, compared to uninfected HEF. However, the expression of these adhesion molecules decreased on herpes simplex virus (HSV)-1 and varicella-zoster virus (VZV)-infected HEF. Increased expression was not observed on HEF treated either with inactivated CMV or with supernatant fluid of CMV-infected cells. The addition of anti-cytokine (TNF-alpha, IL-1 beta, or IFN-gamma) antibodies had no effect on the increase of these adhesion molecules. This suggests that the increase in CD54, CD29, and CD44 on CMV-infected cells requires active virus replication and was not mediated by a soluble factor released from CMV-infected cells. Changes in adhesion molecules on CMV-infected fibroblasts may contribute to inflammation induced by CMV infection.