CD4 memory T cells divide poorly in response to antigen because of their cytokine profile
CD4 memory T cells divide poorly in response to antigen because of their cytokine profile
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DOI：
10.1073/pnas.0807449105
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发表时间：
2008-09-23
影响因子：
11.1
通讯作者：
Marrack, Philippa
中科院分区：
文献类型：
--
作者：
MacLeod, Megan K. L.;McKee, Amy;Marrack, Philippa
免疫记忆是适应性免疫的一个标志，了解T细胞记忆将是开发有效的细胞介导疫苗的核心。CD4记忆细胞的特性和功能尚未明确。在这里，我们证明了二次反应的增加仅仅是记忆池中抗原特异性前体频率增加的结果。记忆细胞比原代应答细胞增殖少，即使在同一宿主内也是如此。通过分析原代细胞和记忆细胞进入细胞周期的情况，我们发现两个群体在第5天之前增殖相似；在这段时间之后，重新激活的记忆细胞增殖的减少了。此时，与原代应答细胞相比，重新激活的记忆细胞中产生IL-2的细胞较少，但产生IFN γ的细胞较多。这两个因素都影响了记忆细胞的低增殖，因为外源性IL-2或抑制IFN γ增加了记忆细胞的增殖。
Immunological memory is a hallmark of adaptive immunity, and understanding T cell memory will be central to the development of effective cell-mediated vaccines. The characteristics and functions of CD4 memory cells have not been well defined. Here we demonstrate that the increased size of the secondary response is solely a consequence of the increased antigen-specific precursor frequency within the memory pool. Memory cells proliferated less than primary responding cells, even within the same host. By analyzing the entry of primary and memory cells into the cell cycle, we found that the two populations proliferated similarly until day 5; after this time, fewer of the reactivated memory cells proliferated. At this time, fewer of the reactivated memory cells made IL-2 than primary responding cells, but more made IFN gamma. Both these factors affected the low proliferation of the memory cells, because either exogenous IL-2 or inhibition of IFN gamma increased the proliferation of the memory cells.