Surface plasmon-coupled emission with gold films
Surface plasmon-coupled emission with gold films
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DOI：
10.1021/jp040221h
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发表时间：
2004-08-19
影响因子：
3.3
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
Gryczynski, I;Malicka, J;Lakowicz, JR
在最近的一份报告中，我们证明了有效的收集发射耦合到表面等离子体上的薄银膜，导致在玻璃基板中的定向信号。我们称这种现象为表面等离子体激元耦合发射（SPCE）。在本报告中，我们研究了磺酰罗丹明101（S101）在薄的聚合物膜上的50 nm厚的金膜玻璃。我们观察到有效的SPCE通过薄的金膜。该结果是令人惊讶的，因为金属金通常是荧光的有效淬灭剂。能量通过金膜以一个清晰的角度有效地耦合到玻璃中，但比可比较的银膜的角度稍不清晰。无论是直接激发还是通过等离子体共振倏逝波激发，约50%的总发射出现为SPCE。发射是p偏振的，不同的波长出现在不同的角度。S101的寿命几乎不受金膜的影响。这些结果表明，SPCE发生在长距离，大于淬火的能量转移到金。我们得出结论，高效的检测设备可以通过使用荧光团的镀金表面。
In a recent report we demonstrated efficient collection of emission by coupling to surface plasmons on a thin silver film, resulting in a directional signal in the glass substrate. We call the phenomenon surface plasmon coupled emission (SPCE). In the present report we examined sulforhodamine 101 (S101) in thin polymer films on 50 nm thick gold films on glass. We observed efficient SPCE through thin gold films. This result was surprising because metallic gold is typically an efficient quencher of fluorescence. The energy effectively coupled through the gold film into the glass at a sharply defined angle, but somewhat less sharp than for a comparable silver film. About 50% of the total emission appeared as SPCE, irrespective of direct excitation or excitation via the plasmon resonance evanescent wave. The emission was p-polarized with different wavelengths appearing at different angles. The lifetime of S101 was mostly unaffected by the gold film. These results indicate that SPCE occurs over long distances, larger than for quenching by energy transfer to the gold. We conclude highly efficient detection devices can be constructed by using fluorophores on gold-coated surfaces.