The apoplastic antioxidant system in Prunus:: response to long-term plum pox virus infection
The apoplastic antioxidant system in Prunus:: response to long-term plum pox virus infection
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DOI：
10.1093/jxb/erl138
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发表时间：
2006-11-01
影响因子：
6.9
通讯作者：
Hernandez, J. A.
中科院分区：
文献类型：
--
作者：
Diaz-Vivancos, P.;Rubio, M.;Hernandez, J. A.
这项工作描述了，第一次，发生在叶质外体的抗氧化系统的变化，以响应李痘病毒（PPV）在不同的李属物种表现出不同的亲和性PPV。桃和杏叶片质外体中存在对羟汞苯甲酸（pHMB）敏感的抗坏血酸过氧化物酶（APX）（class I APX）和对pHMB不敏感的APX（class III APX）、超氧化物歧化酶（SOD）、过氧化物酶（POX）、NADH-POX和多酚氧化酶（PPO）。PPV感染产生不同的变化，从李属植物的叶质外体的抗氧化系统，这取决于他们对病毒的易感性。在极感病的桃品种GF 305中，PPV引起I类APX、POX、NADH-POX和PPO活性的增加。在感病杏品种真实的菲诺，PPV感染产生了质外体POX和SOD活性下降，而PPO的强烈增加观察。然而，在抗杏品种斯塔克早橙子，类APX上升以及POX和SOD活性的强烈增加，注意到在质外体室。长期的PPV感染产生了氧化应激的质外体空间杏和桃植物，观察到的H2 O2含量增加，在这个车厢。然而，这种增加是高得多的PPV易感植物比在抗性杏品种。只有在PPV敏感的杏和桃植物的质外体H2 O2水平的增加伴随着电解质渗漏的增加。在PPV接种的抗性杏叶片中没有观察到电解质渗漏的变化，虽然质外体H2 O2水平增加了42%。双向电泳分析表明，大多数的多肽质外体液中的等电点范围内的pI 4-6。利用MALDI-TOF（基质辅助激光解吸/电离-飞行时间）和肽质量指纹分析的蛋白质鉴定表明，由于PPV感染，桃质外体中的thaumatin样蛋白的诱导以及扁桃腈裂解酶的减少。然而，大多数选择的多肽显示与已知蛋白质没有同源性。这一事实强调，至少在李属中，质外体空间的大部分功能仍然未知。它的结论是，长期的PPV感染产生的氧化应激在叶质外体，PPV感染接种，敏感的李属植物的叶片中产生的有害影响。
This work describes, for the first time, the changes taking place in the antioxidative system of the leaf apoplast in response to plum pox virus (PPV) in different Prunus species showing different susceptibilities to PPV. The presence of p-hydroxymercuribenzoic acid (pHMB)-sensitive ascorbate peroxidase (APX) (class I APX) and pHMB-insensitive APX (class III APX), superoxide dismutase (SOD), peroxidase (POX), NADH-POX, and polyphenoloxidase (PPO) was described in the apoplast from both peach and apricot leaves. PPV infection produced different changes in the antioxidant system of the leaf apoplast from the Prunus species, depending on their susceptibility to the virus. In leaves of the very susceptible peach cultivar GF305, PPV brought about an increase in class I APX, POX, NADH-POX, and PPO activities. In the susceptible apricot cultivar Real Fino, PPV infection produced a decrease in apoplastic POX and SOD activities, whereas a strong increase in PPO was observed. However, in the resistant apricot cultivar Stark Early Orange, a rise in class I APX as well as a strong increase in POX and SOD activities was noticed in the apoplastic compartment. Long-term PPV infection produced an oxidative stress in the apoplastic space from apricot and peach plants, as observed by the increase in H2O2 contents in this compartment. However, this increase was much higher in the PPV-susceptible plants than in the resistant apricot cultivar. Only in the PPV-susceptible apricot and peach plants was the increase in apoplastic H2O2 levels accompanied by an increase in electrolyte leakage. No changes in the electrolyte leakage were observed in the PPV-inoculated resistant apricot leaves, although a 42% increase in the apoplastic H2O2 levels was produced. Two-dimensional electrophoresis analyses revealed that the majority of the polypeptides in the apoplastic fluid had isoelectric points in the range of pI 4-6. The identification of proteins using MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and peptide mass fingerprinting analyses showed the induction of a thaumatin-like protein as well as the decrease of mandelonitrile lyase in peach apoplast due to PPV infection. However, most of the selected polypeptides showed no homology with known proteins. This fact emphasizes that, at least in Prunus, most of the functions of the apoplastic space remain unknown. It is concluded that long-term PPV infection produced an oxidative stress in the leaf apoplast, contributing to the deleterious effects produced by PPV infection in leaves of inoculated, susceptible Prunus plants.