Cis-trans test shows a functional relationship between non-allelic lethal mutations in the T/t-complex.
Cis-trans test shows a functional relationship between non-allelic lethal mutations in the T/t-complex.
复制标题
顺反测试显示 T/t 复合体中非等位基因致死突变之间的功能关系。
DOI：
10.1016/0092-8674(83)90035-1
复制
发表时间：
1983
期刊：
影响因子：
64.5
通讯作者：
Bennett,D
中科院分区：
文献类型：
--
作者：
Shin,HS;McCormick,P;Artzt,K;Bennett,D
小鼠T/t复合体中的隐性致死突变特征性地显示有缺陷的遗传互补，即使它们影响胚胎发生的非常不同的阶段并且已知是非等位的。为了解决它们的遗传或功能关系的问题，我们应用了顺反测试，使用几个定义明确的重组t染色体，携带两个或更多的致命突变，和其他没有特定的致命。我们在这里表明，不同的t-致死之间发生的缺陷互补是一个特定的反式配置的结果，因此这些基因，这可能会映射多达15厘米，构成一个功能单位。一些猜测提出解释这个谜的DNA可塑性。
Recessive lethal mutations in the T/t-complex of the mouse characteristically show defective genetic complementation, even when they affect very different stages of embryogenesis and are known to be nonallelic. To address the question of their genetic or functional relationship, we have applied the cisfrans test, using several well defined recombinant tchromosomes that carry two or more lethal mutations, and others that are devoid of specific lethals. We show here that the defective complementation that occurs between different t-lethals is a specific result of the trans configuration; thus these genes, which may map as much as 15 CM apart, constitute a functional unit. Some speculations are presented to interpret this enigma in terms of DNA plasticity.