Genetic studies on resistance of rice plant to blast fungus IV. Linkage analysis of four genes,Pi-a,Pi-k,Pi-z and Pi-i.:IV. Linkage Analysis of Four Genes, Pi - a , Pi - k , Pi - z and Pi - i
Genetic studies on resistance of rice plant to blast fungus IV. Linkage analysis of four genes,Pi-a,Pi-k,Pi-z and Pi-i.:IV. Linkage Analysis of Four Genes, Pi - a , Pi - k , Pi - z and Pi - i
复制标题
水稻抗稻瘟菌遗传研究IV．
DOI：
10.3186/jjphytopath.47.252
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
A. A. Baluch
中科院分区：
文献类型：
--
作者：
I. Goto;Yeong;A. A. Baluch
日本水稻的连锁图谱揭示了其详细的结构，并确定了几个抗稻瘟病基因的准确位置。本研究报告了两个课题，一个是第8连锁群中Pi-a和Pi-k的线性顺序的校正，另一个是第1连锁群中Pi-i与Pi-z的独立性的验证。六个品种在括号中注明了它们的分析基因，如下：Li-Chi-Chiang（Pi-k），Aichiasahi（Pi-a），Yukara（Pi-a，Pi-k），Yununishiki（Pi-z），Fukei 73（Pi-z）和Fujisaka 5（Pi-i）。通过M博士的仁慈，提供了五名测试人员。北海道大学的Takahashi和T. H-52（A，C），H-69（fs），H-137（fs），Tester 1（fs，Hla）和Tester 2（dt，la），后两个系暂命名为Tester 1，其余系为北海道大学的Tester。其中那牙87、Hoku 1、Ken 53-33和Ken 54-04对5个参试菌株均具有致病力，可分别用于分析Pi-a、Pi-k、Pi-z和Pi-i 4个抗病基因。采用喷雾接种和鞘内接种两种接种方法。表1和表2的每个基因组合中总结了许多试验结果，其中观察到的性状在Fz群体中符合单基因分离3：1。根据表1，四个基因Pi-a-la-dt-Pi-k以上述顺序排列在第8连锁群上，其各自的重组值如图1所示。推测该群体对应于第9号染色体1，2），并确定这3个基因sp_（1），la_（1）和Pi-a_（3）与九州大学易位纯合体系RT_（7-9）的断裂点紧密连锁，如图1所示。在同一张地图上，dt 4）位于sp的背面，横跨la。因此，本研究中假设的Pi-a和Pi-k的线性顺序是合理的。与目前所接受的方案相比，第8组中这两个抗稻瘟病基因的排列顺序正好相反，可能是以前用H-68标记sh不适合分析
The linkage map in Japanese rice has revealed its details and confirmed the accurate position of several blast resistance genes. Two subjects were reported in the present study, the one was rectified linear order of Pi-a and Pi-k in the 8th linkage group and the other verification of independence of Pi-i from Pi-.z of the 1st group. Six cultivars were noted with their analyzed gene (s) in parenthesis as follows; Li-Chi-Chiang (Pi-k), Aichiasahi (Pi-a), Yukara (Pi-a, Pi-k), Fukunishiki (Pi-z), Fukei 73 (Pi-z) and Fujisaka 5 (Pi-i). Five testers were made available through kindness of Dr. M. Takahashi of Hokkaido Univ. and of Dr. T. Omura of Kyushu Univ. as follows; H-52 (A, C), H-69 (fs), H-137 (fs), Tester 1 (fs, Hla) and Tester 2 (dt, la), the later 2 lines were tentatively named in the present study and the others were testers of Hokkaido University. Four isolates used, Naga 87, Hoku 1, Ken 53-33 and Ken 54-04, were virulent to all these 5 testers and valid to analyze the present 4 resistance genes, Pi-a, Pi-k, Pi-z and Pi-i, respectively. The spray and sheath inoculation methods were carried out. Many experimental results, in which observed characters fitted to the monogenic segrega tion 3: 1 in Fz populations, were summarized in each gene combination of Tables 1 and 2. The rectified linear order of Pi-a and Pi-k According to Table 1, four genes, Pi-a-la-dt-Pi-k, were arranged in the order above mentioned on the 8 th linkage group with respective recombination value as shown in Fig. 1. The present group was estimated to correspondent to the 9th chromosome1,2) and these three genes, sp1), la1) and Pi-a3), were acertained to be closely linked with the break-point of RT 7-9, one of translocation homozygote lines of Kyushu Univ., as shown on Fig. 1. On the same map, dt4) was situated on the reverse side of sp across la. On these accounts the linear order of Pi-a and Pi-k postulated in the present study was reasonable. As compare with the proposal hitherto accepted5), the order of these two blast resistance genes was to the contrary in the 8th group, perhaps H-68 previously used to mark sh was not appropriate to analyze