The functional subunit of a dimeric transcription activator protein depends on promoter architecture.
The functional subunit of a dimeric transcription activator protein depends on promoter architecture.
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二聚体转录激活蛋白的功能亚基取决于启动子结构。
DOI：
10.1002/j.1460-2075.1994.tb06776.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Ebright,RH
中科院分区：
文献类型：
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作者：
Zhou,Y;Pendergrast,PS;Bell,A;Williams,R;Busby,S;Ebright,RH
在I类CAP依赖的启动子中，CAP的DNA位点位于RNA聚合酶的DNA位点的上游。在依赖第二类CAP的启动子中，CAP的DNA位点与RNA聚合酶的DNA位点重叠，取代了-35位点。我们用“定向异源二聚体”方法鉴定了CAP功能亚基在两个第I类启动子和一个第II类启动子[CC(-61.5)和CC(-72.5)]和一个第II类启动子上。我们的结果表明，在I类启动子上的转录激活，无论CAP和RNA聚合酶的DNA位点之间的距离有多远，都需要CAP启动子-近端亚基的激活区。与之形成鲜明对比的是，我们的结果表明，在II类启动子上的转录激活需要CAP启动子-远端亚单位的激活区。
In Class I CAP‐dependent promoters, the DNA site for CAP is located upstream of the DNA site for RNA polymerase. In Class II CAP‐dependent promoters, the DNA site for CAP overlaps the DNA site for RNA polymerase, replacing the ‐35 site. We have used an ‘oriented heterodimers’ approach to identify the functional subunit of CAP at two Class I promoters having different distances between the DNA sites for CAP and RNA polymerase [CC(‐61.5) and CC(‐72.5)] and at one Class II promoter [CC(‐41.5)]. Our results indicate that transcription activation at Class I promoters, irrespective of the distance between the DNA sites for CAP and RNA polymerase, requires the activating region of the promoter‐proximal subunit of CAP. In striking contrast, our results indicate that transcription activation at Class II promoters requires the activating region of the promoter‐distal subunit of CAP.
“1972政权”日本安保体系下的台湾（中文）
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国：EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者：
福田円